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Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation
Abstract
The positive effect of low-magnitude, high‑frequency (LMHF) vibration on implant osseointegration has been demonstrated; however, the underlying cellular and molecular mechanisms remain unknown. The aim of this study was to explore the effect of LMHF vibration on the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) cultured on hydroxyapatite (HA)-coated surfaces in an in vitro model as well as to elucidate the molecular mechanism responsible for the effects of LMHF vibration on osteogenesis. LMHF vibration resulted in the increased expression of fibronectin, which was measured by immunostaining and RT-qPCR. Stimulation of BMSCs by LMHF vibration resulted in the rearrangement of the actin cytoskeleton with more prominent F-actin. Moreover, the expression of β1 integrin, vinculin and paxillin was notably increased following LMHF stimulation. Scanning electron microscope observations revealed that there were higher cell numbers and more extracellular matrix attached to the HA-coated surface in the LMHF group. Alkaline phosphatase activity as well as the expression of osteogenic-specific genes, namely Runx2, osterix, collagen I and osteocalcin, were significantly elevated in the LMHF group. In addition, the protein expression of Wnt10B, β-catenin, Runx2 and osterix was increased following exposure to LMHF vibration. Taken together, the findings of this study indicate that LMHF vibration promotes the adhesion and the osteogenic differentiation of BMSCs on HA-coated surfaces in vitro, and LMHF vibration may directly induce osteogenesis by activating the Wnt/β‑catenin signaling pathway. These data suggest that LMHF vibration enhances the osseointegration of bone to a HA-coated implant, and provide a scientific foundation for improving bone-implant osseointegration through the application of LMHF vibration.
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“Hydroxyapatite, a natural apatite mineral, has the capacity to interact with bone marrow mesenchymal stem cells (BMSCs) and enhance BMSC proliferation, differentiation, and mineralization through its action on various signaling pathways, including ERK/Sox9, BMP/Smad, Wnt/β-catenin, TGF-β, and MAPK [64,65,66,67,68].”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [66]
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Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review
Zhu, S., Zhang, Q., Xu, X., Liu, Z., Cheng, G., Long, D., Cheng, L., & Dai, F. (2025) · Polymers · doi:10.3390/polym17060772 · Cited by 36
Metadata
- Authors
- Chen, B., Lin, T., Yang, X., Li, Y., Xie, D., Zheng, W., Cui, H., Deng, W., & Tan, X.
- Affiliations
- The First Affiliated Hospital, Sun Yat-sen University (CN)
- Chinese PLA General Hospital (CN)
- Guangzhou Women and Children Medical Center (CN)
- Third Affiliated Hospital of Southern Medical University (CN)
- Sun Yat-sen University (CN)
- General Hospital of Guangzhou Military Command (CN)
- ORCID
- Venue
- International Journal of Molecular Medicine
- Publisher
- Spandidos Publications
- Published
- 2016-09-28
- Volume
- 38
- Issue
- 5
- Pages
- 1531-1540
- ISSN
- 1107-3756 (ISSN-L)
- 1791-244X
- OpenAlex
- W2526918269
- Cited by
- 42
- References
- 51
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 6 · 2020: 3 · 2019: 4 · 2018: 4 · 2017: 1
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Molecular Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Effects of Vibration on Health
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- low-magnitude high-frequency vibration, bone marrow-derived mesenchymal stem cells, osteogenic differentiation, cell adhesion, hydroxyapatite-coated implants, Wnt/β-catenin signaling, osseointegration, beta1 integrin, actin cytoskeleton, Runx2, Osterix, extracellular matrix, fibronectin
- MeSH terms
- Alkaline Phosphatase, Animals, Bone Marrow Cells, Cell Adhesion, Cell Differentiation, Cells, Cultured, Cytoskeleton, Extracellular Matrix, Fibronectins, Fluorescent Antibody Technique, Gene Expression Regulation, Male, Osteogenesis, RNA, Messenger, Vibration, Rats, Sprague-Dawley, Durapatite, Coated Materials, Biocompatible, Cell Shape, Cell Proliferation, Mesenchymal Stem Cells, Wnt Signaling Pathway, Real-Time Polymerase Chain Reaction
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