CoChat
Sign Up
Cited by 58Osteoarthritis Treatment and MechanismsCurcumin's Biomedical ApplicationsAdvanced Drug Delivery Systems

Therapeutic potential of hyaluronic acid/chitosan nanoparticles for the delivery of curcuminoid in knee osteoarthritis and an in�vitro evaluation in chondrocytes

Open access
Wang, Jian, Wang, Xiang, Cao, Yun, Huang, Tao, Song, Deng‑Xin, Tao, Hai‑Rong·2018·International Journal of Molecular Medicine
DOI 10.3892/ijmm.2018.3817PMC PMC6192775
Open this paper in CoChat — free accountOpen PDF

Abstract

Knee osteoarthritis (OA) is the main cause of leg pain in middle‑aged and elderly individuals. Hyaluronic acid (HA), as well as curcuminoid, has been used in the treatment of knee OA. In the present study, HA/chitosan nanoparticles (CNPs) were prepared for the delivery of curcuminoid, in order to investigate whether HA and curcuminoid can act synergistically as a better treatment option. The knee OA model was established by the Hulth method, and a knee OA chondrocyte model was constructed by the co‑induction of interleukin‑1β and tumor necrosis factor (TNF)‑α. The drug loading capacity of HA/CNP for the delivery of curcuminoid was measured by an ultraviolet assay, and the cytotoxicity to chondrocytes was measured by an MTT assay. Collagen II was detected by immunofluorescence, and the expression levels of nuclear factor (NF)‑κB and inflammation‑related genes in cartilage tissue and chondrocytes were detected. Chondrocyte proliferation was determined by an EdU assay, and chondrocyte apoptosis was determined by flow cytometry. The Mankin pathological score of the Outerbridge classification was obtained. The results demonstrated that the optimum drug loading capacity of HA/CNP for the delivery of curcuminoid was 38.44%, with a good sustained release function. HA/CNP treatment resulted in inhibition of the NF‑κB pathway, as well as the expression of matrix metalloproteinase (MMP)‑1 and MMP‑13, but it increased collagen II expression. HA/CNP for the delivery of curcuminoid significantly decreased the Outerbridge classification and Mankin pathological scores to close to normal until the 4th week. Furthermore, it was also observed that all the effects of HA/CNP on the delivery of curcuminoid were more prominent compared with the effects of HA or curcuminoid treatment individually. Taken together, these findings demonstrated that HA/CNP for the delivery of curcuminoid may suppress inflammation and chondrocyte apoptosis in knee OA via repression of the NF‑κB pathway.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3892/ijmm.2018.3817

Cites

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

No references could be resolved yet.

Research works printed but not linked: 29

  1. [1]Wu Q Sun X Du L Association of fibulin-3 concentrations with the presence and severity of knee osteoarthritis: A cross-sectional study Knee 24 1369 1373 2017 10.1016/j.knee.2017.04.018 29195846 · DOI
  2. [2]Ogunbode AM Adebusoye LA Olowookere OO Alonge TO Physical functionality and self-rated health status of adult patients with knee osteoarthritis presenting in a primary care clinic Ethiop J Health Sci 24 319 328 2014 10.4314/ejhs.v24i4.7 25489196 PMC4248031 · DOI
  3. [3]Runhaar J van Middelkoop M Reijman M Willemsen S Oei EH Vroegindeweij D van Osch G Koes B Bierma-Zeinstra SM Prevention of knee osteoarthritis in overweight females: The first preventive randomized controlled trial in osteoarthritis Am J Med 128 888 895.e4 2015 10.1016/j.amjmed.2015.03.006 25818496 · DOI
  4. [4]Ji B Guo W Ma H Xu B Mu W Zhang Z Amat A Cao L Isoliquiritigenin suppresses IL-1β induced apoptosis and inflammation in chondrocyte-like ATDC5 cells by inhibiting NF-κB and exerts chondroprotective effects on a mouse model of anterior cruciate ligament transection Int J Mol Med 40 1709 1718 2017 29039445 PMC5716454
  5. [5]Ricci M Micheloni GM Berti M Perusi F Sambugaro E Vecchini E Magnan B Clinical comparison of oral administration and viscosupplementation of hyaluronic acid (HA) in early knee osteoarthritis Musculoskelet Surg 101 45 49 2017 10.1007/s12306-016-0428-x 27681813 · DOI
  6. [6]Zhang Z Leong DJ Xu L He Z Wang A Navati M Kim SJ Hirsh DM Hardin JA Cobelli NJ Curcumin slows osteoarthritis progression and relieves osteoarthritis-associated pain symptoms in a post-traumatic osteoarthritis mouse model Arthritis Res Ther 18 128 2016 10.1186/s13075-016-1025-y 27260322 PMC4891896 · DOI
  7. [7]Salwowska NM Bebenek KA Żądło DA Wcisło- Dziadecka DL Physiochemical properties and application of hyaluronic acid: A systematic review J Cosmet Dermatol 15 520 526 2016 10.1111/jocd.12237 27324942 · DOI
  8. [8]Concoff A Sancheti P Niazi F Shaw P Rosen J The efficacy of multiple versus single hyaluronic acid injections: A systematic review and meta-analysis BMC Musculoskelet Disord 18 542 2017 10.1186/s12891-017-1897-2 29268731 PMC5740709 · DOI
  9. [9]Li X Feng K Li J Yu D Fan Q Tang T Yao X Wang X Curcumin inhibits apoptosis of chondrocytes through activation ERK1/2 signaling pathways induced autophagy Nutrients 9 E414 2017 10.3390/nu9040414 28430129 PMC5409753 · DOI
  10. [10]Wang F Yang Y Ju X Udenigwe CC He R Polyelectrolyte complex nanoparticles from chitosan and acylated rapeseed cruciferin protein for curcumin delivery J Agric Food Chem 66 2685 2693 2018 10.1021/acs.jafc.7b05083 29451796 · DOI
  11. [11]Yu H Nguyen MH Hadinoto K Effects of chitosan molecular weight on the physical and dissolution characteristics of amorphous curcumin-chitosan nanoparticle complex Drug Dev Ind Pharm 44 82 88 2018 10.1080/03639045.2017.1373802 28849675 · DOI
  12. [12]Yasuda T Type II collagen peptide stimulates Akt leading to nuclear factor-kappaB activation: Its inhibition by hyaluronan Biomed Res 35 193 199 2014 10.2220/biomedres.35.193 24942858 · DOI
  13. [13]Yang Q Wu S Mao X Wang W Tai H Inhibition effect of curcumin on TNF-α and MMP-13 expression induced by advanced glycation end products in chondrocytes Pharmacology 91 77 85 2013 10.1159/000345345 23183190 · DOI
  14. [14]Zhou L Hu Y Li C Yan Y Ao L Yu B Fang W Liu J Li Y Levocorydalmine alleviates vincristine-induced neuropathic pain in mice by inhibiting an NF-kappa B-dependent CXCL1/CXCR2 signaling pathway Neuropharmacology 135 34 47 2018 10.1016/j.neuropharm.2018.03.004 29518397 · DOI
  15. [15]Xu H Bouta EM Wood RW Schwarz EM Wang Y Xing L Utilization of longitudinal ultrasound to quantify joint soft-tissue changes in a mouse model of posttraumatic osteoarthritis Bone Res 5 17012 2017 10.1038/boneres.2017.12 28638676 PMC5468547 · DOI
  16. [16]Xu J Lu MX Cui YD Du YZ Selection and evaluation of reference genes for expression analysis using qRT-PCR in Chilo suppressalis (Lepidoptera: Pyralidae) J Econ Entomol 110 683 691 2017 28115499
  17. [17]Xu Y Asghar S Yang L Chen Z Li H Shi W Li Y Shi Q Ping Q Xiao Y Nanoparticles based on chitosan hydrochloride/hyaluronic acid/PEG containing curcumin: In vitro evaluation and pharmacokinetics in rats Int J Biol Macromol 102 1083 1091 2017 10.1016/j.ijbiomac.2017.04.105 28472690 · DOI
  18. [18]Chen C Zhang C Cai L Xie H Hu W Wang T Lu D Chen H Baicalin suppresses IL-1β-induced expression of inflammatory cytokines via blocking NF-κB in human osteoarthritis chondrocytes and shows protective effect in mice osteoarthritis models Int Immunopharmacol 52 218 226 2017 10.1016/j.intimp.2017.09.017 28942223 · DOI
  19. [19]Sharma M Sahu K Singh SP Jain B Wound healing activity of curcumin conjugated to hyaluronic acid: In vitro and in vivo evaluation Artif Cells Nanomed Biotechnol 46 1009 1017 2018 10.1080/21691401.2017.1358731 28754055 · DOI
  20. [20]Zhuang Z Ye G Huang B Kaempferol alleviates the interleukin-1β-induced inflammation in rat osteoarthritis chondrocytes via suppression of NF-κB Med Sci Monit 23 3925 3931 2017 10.12659/MSM.902491 28806392 PMC5566200 · DOI
  21. [21]De Palma A Cheleschi S Pascarelli NA Giannotti S Galeazzi M Fioravanti A Hydrostatic pressure as epigenetic modulator in chondrocyte cultures: A study on miRNA-155, miRNA-181a and miRNA-223 expression levels J Biomech 66 165 169 2018 10.1016/j.jbiomech.2017.10.044 29150345 · DOI
  22. [22]Li Y Wang J Song X Bai H Ma T Zhang Z Li X Jiang R Wang G Fan X Effects of baicalein on IL-1β-induced inflammation and apoptosis in rat articular chondrocytes Oncotarget 8 90781 90795 2017 29207603 PMC5710884
  23. [23]Hwang HS Kim HA Chondrocyte apoptosis in the pathogenesis of osteoarthritis Int J Mol Sci 16 26035 26054 2015 10.3390/ijms161125943 26528972 PMC4661802 · DOI
  24. [24]Lee HG Yang JH PCB126 induces apoptosis of chondrocytes via ROS-dependent pathways Osteoarthritis Cartilage 20 1179 1185 2012 10.1016/j.joca.2012.06.004 22750746 · DOI
  25. [25]Ito K Shinomura T Development and application of a new Silent reporter system to quantitate the activity of enhancer elements in the type II collagen gene Gene 585 13 21 2016 10.1016/j.gene.2016.03.019 26992640 · DOI
  26. [26]Motomura H Seki S Shiozawa S Aikawa Y Nogami M Kimura T A selective c-Fos/AP-1 inhibitor prevents cartilage destruction and subsequent osteophyte formation Biochem Biophys Res Commun 497 756 761 2018 10.1016/j.bbrc.2018.02.147 29476740 · DOI
  27. [27]Stancker TG Vieira SS Serra AJ do Nascimento Lima R Dos Santos Feliciano R Silva JA Jr Dos Santos SA Dos Santos Vieira MA Simões MCB Leal-Junior EC Can photobiomodulation associated with implantation of mesenchymal adipose-derived stem cells attenuate the expression of MMPs and decrease degradation of type II collagen in an experimental model of osteoarthritis Lasers Med Sci 33 1073 1084 2018 10.1007/s10103-018-2466-0 29520686 · DOI
  28. [28]Pohlig F Guell F Lenze U Lenze FW Mühlhofer HM Schauwecker J Toepfer A Mayer-Kuckuk P von Eisenhart-Rothe R Burgkart R Salzmann GM Hyaluronic acid suppresses the expression of metalloproteinases in osteoarthritic cartilage stimulated simultaneously by interleukin 1β and mechanical load PLoS One 11 e0150020 2016 10.1371/journal.pone.0150020 26934732 PMC4774918 · DOI
  29. [29]Li WQ Dehnade F Zafarullah M Oncostatin M-induced matrix metalloproteinase and tissue inhibitor of metalloproteinase-3 genes expression in chondrocytes requires Janus kinase/STAT signaling pathway J Immunol 166 3491 3498 2001 10.4049/jimmunol.166.5.3491 11207308 · DOI

Cited by

Sorted by citations

Authors
Wang, J., Wang, X., Cao, Y., Huang, T., Song, D., & Tao, H.
Affiliations
  • Shanghai First People's Hospital (CN)
  • Shanghai Jiao Tong University (CN)
  • Department of Orthopedics, Baoshan Branch of Shanghai First People's Hospital, Shanghai 200940, P.R. China
  • Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, P.R. China
ORCID
Venue
International Journal of Molecular Medicine
Publisher
Spandidos Publications
Published
2018-08-09
Volume
42
Issue
5
Pages
2604-2614
ISSN
  • 1107-3756 (ISSN-L)
  • 1791-244X
DOI
10.3892/ijmm.2018.3817
PMC
PMC6192775
OpenAlex
W2886128255
Cited by
58
References
0
Citations by year
2025: 6 · 2024: 10 · 2023: 8 · 2022: 14 · 2021: 9 · 2020: 9 · 2019: 1 · 2017: 1
FWCI
3.96
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • International Journal of Molecular Medicine (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Curcumin's Biomedical Applications
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
Keywords
knee osteoarthritis, hyaluronic acid, chitosan nanoparticles, curcuminoids, drug delivery, chondrocytes, NF-κB signaling, chondrocyte apoptosis, cartilage inflammation, type II collagen, MMP-1, matrix metalloproteinase-13, drug loading capacity, sustained drug release, IL-1β, TNF-α
MeSH terms
Animals, Curcumin, Flow Cytometry, Fluorescent Antibody Technique, Hyaluronic Acid, Male, Blotting, Western, Rats, Sprague-Dawley, Chondrocytes, Reverse Transcriptase Polymerase Chain Reaction, Osteoarthritis, Knee, Matrix Metalloproteinase 1, Collagen Type II, Chitosan, Rats, Matrix Metalloproteinase 13, Nanoparticles

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review