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Cited by 20Connexins and lens biologyAntioxidant Activity and Oxidative StressIntraocular Surgery and Lenses

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L‑carnitine alleviates oxidative stress‑related damage via MAPK signaling in human lens epithelial cells exposed to H2O2

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Li, Xiaoxia, Meng, Fanlan, Li, Hua, Hua, Xia, Wu, Li'an, Yuan, Xiaoyong·2019·International Journal of Molecular Medicine
DOI 10.3892/ijmm.2019.4283
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Abstract

L‑carnitine (LC) is well known for its antioxidative properties. The present study aimed to evaluate the effects of LC on human lens epithelial cells (HLECs) and to analyze its regulatory mechanism in cataractogenesis. HLE B‑3 cells were cultured with hydrogen peroxide (H2O2) and were pretreated with or without LC. The Cell Counting kit‑8 assay was used to determine cell viability. Reactive oxygen species (ROS) assay kit was used to measure the cellular ROS production induced by H2O2 and LC. In addition, reverse transcription‑quantitative PCR and western blot analysis were performed to detect the expression levels of oxidative damage markers and antioxidant enzymes. Notably, ROS overproduction was observed upon exposure to H2O2, whereas LC supplementation markedly decreased ROS levels through activation of the antioxidant enzymes forkhead box O1, peroxiredoxin 4 and catalase. Furthermore, LC suppressed the expression of apoptosis‑associated genes (caspase-3) and inflammation‑associated genes [interleukin (IL)1, IL6, IL8 and cyclooxygenase‑2]. Conversely, LC promoted proliferating cell nuclear antigen, cyclin‑dependent kinase (CDK)2 and CDK4 expression, which may increase proliferation of HLECs that were incubated with H2O2. In addition, epithelial‑mesenchymal transition occurred upon ROS accumulation, whereas the effects of H2O2 on AQP1 and vimentin expression were reversed upon LC supplementation. Notably, this study revealed that LC restored the oxidant/antioxidant balance and protected against cell damage through the mitogen‑activated protein kinase signaling pathway. In conclusion, LC may serve a protective role in curbing oxidative damage and therefore may be considered a potential therapeutic agent for the treatment of cataracts.

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References 33 · Cited by 20

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  1. Nagai, 2011, Correlation between hyper-sensitivity to hydrogen peroxide and low defense against Ca(2+) influx in cataractogenic lens of Ihara cataract rats, Biol Pharm Bull, 34, 1005, 10.1248/bpb.34.1005 · DOI
  2. Zuercher, 2010, Alterations of the 5′ untranslated region of SLC16A12 lead to age-related cataract, Invest Ophthalmol Vis Sci, 51, 3354, 10.1167/iovs.10-5193 · DOI
  3. Chang, 2013, Serum antioxidative enzymes levels and oxidative stress products in age-related cataract patients, Oxid Med Cell Longev, 2013, 587826, 10.1155/2013/587826 · DOI
  4. Mok, 2014, Antiapoptotic effects of anthocyanin from the seed coat of black soybean against oxidative damage of human lens epithelial cell induced by H2O2, Curr Eye Res, 39, 1090, 10.3109/02713683.2014.903497 · DOI
  5. Fujii, 2015, Physiological and pathological views of peroxiredoxin 4, Free Radic Biol Med, 83, 373, 10.1016/j.freeradbiomed.2015.01.025 · DOI
  6. Yamada, 2018, Peroxiredoxin 4 (PRDX4): Its critical in vivo roles in animal models of metabolic syndrome ranging from atherosclerosis to nonalcoholic fatty liver disease, Pathol Int, 68, 91, 10.1111/pin.12634 · DOI
  7. Kim, 2014, Ginsenoside Rc modulates Akt/FoxO1 pathways and suppresses oxidative stress, Arch Pharm Res, 37, 813, 10.1007/s12272-013-0223-2 · DOI
  8. Kubo, 2018, Roles of TGF β and FGF signals in the lens: Tropomyosin regulation for posterior capsule opacity, Int J Mol Sci, 19, E3093, 10.3390/ijms19103093 · DOI
  9. Lamouille, 2014, Molecular mechanisms of epithelial-mesenchymal transition, Nat Rev Mol Cell Biol, 15, 178, 10.1038/nrm3758 · DOI · Paper page
  10. Schey, 2017, The role of aquaporins in ocular lens homeostasis, Int J Mol Sci, 18, E2693, 10.3390/ijms18122693 · DOI
  11. Dahm, 2004, Dying to see, Sci Am, 291, 82, 10.1038/scientificamerican1004-82 · DOI
  12. Traina, 2016, The neurobiology of acetyl-L-carnitine, Front Biosci (Landmark Ed), 21, 1314, 10.2741/4459 · DOI
  13. Nutrients Editorial Office, 2018, Erratum: l-Carnitine supplementation in recovery after exercise; Nutrients 2018, 10, 349, Nutrients, 10, E541, 10.3390/nu10050541 · DOI
  14. Mishra, 2016, L-carnitine mediated reduction in oxidative stress and alteration in transcript level of antioxidant enzymes in sheep embryos produced in vitro, Reprod Domest Anim, 51, 311, 10.1111/rda.12682 · DOI
  15. Deng, 2014, Osmoprotectants suppress the production and activity of matrix metalloproteinases induced by hyperosmolarity in primary human corneal epithelial cells, Mol Vis, 20, 1243
  16. Mitchell, 2018, The carnitine shuttle pathway is altered in patients with neovascular age-related macular degeneration, Invest Ophthalmol Vis Sci, 59, 4978, 10.1167/iovs.18-25137 · DOI
  17. Livak, 2001, Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method, Methods, 25, 402, 10.1006/meth.2001.1262 · DOI · Paper page
  18. Werner, 2014, Opposite roles for p38MAPK-driven responses and reactive oxygen species in the persistence and resolution of radiation-induced genomic instability, PLoS One, 9, e108234, 10.1371/journal.pone.0108234 · DOI
  19. Bai, 2017, Ghrelin protects human lens epithelial cells against oxidative stress-induced damage, Oxid Med Cell Longev, 2017, 1910450, 10.1155/2017/1910450 · DOI
  20. Akasaki, 2014, FoxO transcription factors support oxidative stress resistance in human chondrocytes, Arthritis Rheumatol, 66, 3349, 10.1002/art.38868 · DOI
  21. Patel, 2016, Induced peroxidase and cytoprotective enzyme expressions support adaptation of HUVECs to sustain subsequent H2O2 exposure, Microvasc Res, 103, 1, 10.1016/j.mvr.2015.09.003 · DOI
  22. Zhu, 2018, The protective effect of beta-casomor-phin-7 via promoting Foxo1 activity and nuclear translocation in human lens epithelial cells, Cutan Ocul Toxicol, 37, 267, 10.1080/15569527.2018.1445095 · DOI
  23. Shamsi, 2007, L-carnitine protects human retinal pigment epithelial cells from oxidative damage, Curr Eye Res, 32, 575, 10.1080/02713680701363833 · DOI
  24. Thrimawithana, 2018, Drug delivery to the lens for the management of cataracts, Adv Drug Deliv Rev, 126, 185, 10.1016/j.addr.2018.03.009 · DOI
  25. Yuan, 2015, Ocular drug delivery nanowafer with enhanced therapeutic efficacy, ACS Nano, 9, 1749, 10.1021/nn506599f · DOI
  26. Zhao, 2016, Molecular cloning and functional characterization of cyclin E and CDK2 from penaeus monodon, Genet Mol Res, 15, 10.4238/gmr.15038716 · DOI
  27. Kanska, 2016, Cyclin E as a potential therapeutic target in high grade serous ovarian cancer, Gynecol Oncol, 143, 152, 10.1016/j.ygyno.2016.07.111 · DOI
  28. Chou, 2017, From the cover: l-Carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial-mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate, Toxicol Sci, 160, 217, 10.1093/toxsci/kfx183 · DOI
  29. Baierle, 2015, Relationship between inflammation and oxidative stress and cognitive decline in the institutionalized elderly, Oxid Med Cell Longev, 2015, 804198, 10.1155/2015/804198 · DOI
  30. Dogru, 2018, Potential role of oxidative stress in ocular surface inflammation and dry eye disease, Invest Ophthalmol Vis Sci, 59, DES163, 10.1167/iovs.17-23402 · DOI
  31. Hua, 2017, ROS-induced oxidative injury involved in pathogenesis of fungal keratitis via p38 MAPK activation, Sci Rep, 7, 10421, 10.1038/s41598-017-09636-w · DOI
  32. Xu, 2018, Ketamine delays progression of oxidative and damaged cataract through regulating HMGB-1/NF-κB in lens epithelial cells, Immunopharmacol Immunotoxicol, 40, 303, 10.1080/08923973.2018.1478851 · DOI
  33. Jia, 2011, Grape seed proanthocyanidin extract protects human lens epithelial cells from oxidative stress via reducing NF-κB and MAPK protein expression, Mol Vis, 17, 210
  34. Yao, 2008, Epigallocatechin gallate protects against oxidative stress-induced mitochondria-dependent apoptosis in human lens epithelial cells, Mol Vis, 14, 217

Metadata

Authors
Li, X., Meng, F., Li, H., Hua, X., Wu, L., & Yuan, X.
Affiliations
  • Tianjin Medical University (CN)
  • Second Hospital of Tianjin Medical University (CN)
  • Xi'an Jiaotong University (CN)
ORCID
Venue
International Journal of Molecular Medicine
Publisher
Spandidos Publications
Published
2019-07-22
Volume
44
Issue
4
Pages
1515-1522
ISSN
  • 1107-3756 (ISSN-L)
  • 1791-244X
DOI
10.3892/ijmm.2019.4283
OpenAlex
W2963081899
Cited by
20
References
33
Citations by year
2025: 4 · 2024: 5 · 2023: 2 · 2022: 5 · 2021: 3 · 2020: 1
FWCI
0.75
Type
journal article
Language
en
Open access
Locations
  • International Journal of Molecular Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81670817)
  • China Postdoctoral Science Foundation (2018M641665)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
  • Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Ophthalmology › Intraocular Surgery and Lenses
Keywords
L-carnitine, cataract, lens epithelial cells, oxidative stress, hydrogen peroxide, reactive oxygen species, MAPK signaling, FOXO1, peroxiredoxin 4, catalase, apoptosis, inflammation, epithelial-mesenchymal transition, cell proliferation, antioxidant enzymes
MeSH terms
Carnitine, Cell Survival, Cells, Cultured, Epithelial Cells, Humans, Hydrogen Peroxide, Lens, Crystalline, Oxidation-Reduction, Biomarkers, Apoptosis, Reactive Oxygen Species, Oxidative Stress, MAP Kinase Signaling System, Apoptosis Regulatory Proteins

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CoChat. (2026). Citation check: L‑carnitine alleviates oxidative stress‑related damage via MAPK signaling in human lens epithelial cells exposed to H2O2. https://app.cochat.ai/papers/10.3892/ijmm.2019.4283
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Li, X., Meng, F., Li, H., Hua, X., Wu, L., & Yuan, X. (2019). L‑carnitine alleviates oxidative stress‑related damage via MAPK signaling in human lens epithelial cells exposed to H2O2. International Journal of Molecular Medicine. https://doi.org/10.3892/ijmm.2019.4283