Ascorbic Acid Provides Protection for Human Chondrocytes against Oxidative Stress
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Oxidative stress is considered to be an important cause of dysfunction in chondrocytes and articular cartilage degradation, which leads to the pathogenesis of osteoarthritis (OA) and cartilage aging. The present study aimed to assess the effects of the widely applied antioxidant, ascorbic acid (AA), on human chondrocytes against hydrogen peroxide (H2O2) in vitro. Using annexin V‑fluorescein isothiocyanate, 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyl tetrazolium bromide and senescence‑associated β‑galactosidase assays, the present study identified that AA reduced apoptosis, reduced the loss of viability and markedly decreased H2O2‑mediated senescence in cells treated with H2O2. Furthermore, AA not only stimulated the expression levels of collagens and proteoglycans, but also inhibited the differentiation of chondrocytes under conditions of oxidative stress. In addition, reverse transcription‑quantitative polymerase chain reaction and western blotting demonstrated that AA decreased the activity of nrf2, NF‑κB, AP1 and matrix metalloproteinase‑3, which is stimulated by H2O2. In conclusion, AA efficiently protected human chondrocytes against damage induced by H2O2 by regulating multiple regulatory pathways.
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Ascorbic acid has a protective effect on human chondrocytes against OS by attenuating the increase of apoptosis, the loss of viability and the increase of senescence, and therefore hindering the development of osteoarthritis and aging of cartilage.
“Moreover, AA was found to have a protective effect also on human chondrocytes against OS by attenuating the increase of apoptosis, the loss of viability and the increase of senescence, and therefore hindering the development of osteoarthritis and aging of cartilage [125,126].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [125]
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References 45 · Cited by 62
- Authors
- CHANG, Z., HUO, L., LI, P., WU, Y., & ZHANG, P.
- Affiliations
- Second Affiliated Hospital of Inner Mongolia Medical University (CN)
- Venue
- Molecular Medicine Reports
- Publisher
- Spandidos Publications
- Published
- 2015-08-20
- Volume
- 12
- Issue
- 5
- Pages
- 7086-7092
- ISSN
- 1791-2997 (ISSN-L)
- 1791-3004
- OpenAlex
- W1946894587
- Cited by
- 62
- References
- 45
- Citations by year
- 2026: 8 · 2025: 5 · 2024: 7 · 2023: 3 · 2022: 7 · 2021: 7 · 2020: 5 · 2019: 5 · 2018: 5 · 2017: 4 · 2016: 6
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Medicine Reports (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Inner Mongolia Medical University
- Topics
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- vitamin C, chondrocytes, oxidative stress, hydrogen peroxide, osteoarthritis, cartilage degeneration, apoptosis, cellular senescence, collagen, proteoglycans, chondrogenic differentiation, Nrf2, NF-κB, AP-1, matrix metalloproteinase-3, antioxidants
- MeSH terms
- Antioxidants, Ascorbic Acid, Cell Line, Cell Survival, Collagen, Humans, Hydrogen Peroxide, Proteoglycans, Cellular Senescence, Apoptosis, Oxidative Stress, Chondrocytes
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