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Cited by 36Bone Metabolism and DiseasesBone health and osteoporosis researchEstrogen and related hormone effects

Effect of β‑ecdysterone on glucocorticoid‑induced apoptosis and autophagy in osteoblasts

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Tang, Yang‑Hua, Yue, Zhen‑Shuang, Li, Guo‑Song, Zeng, Lin‑Ru, Xin, Da‑Wei, Hu, Zhong‑Qing, Xu, Can‑Da·2017·Molecular Medicine Reports
DOI 10.3892/mmr.2017.7840PMC PMC5780097
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Abstract

The aim of the present study was to investigate the effect of glucocorticoids in osteoblasts and to examine the role of β‑ecdysterone in the pathogenesis of glucocorticoid‑induced osteoporosis. Osteoblasts were induced from bone marrow mesenchymal stem cells, which were isolated from C57BL/6 mice. Cell viability and apoptosis of osteoblasts were measured by Cell Counting Kit‑8 and flow cytometry analysis, respectively. The expression of related genes and proteins was measured by reverse transcription quantitative polymerase chain reaction and western blot analysis respectively. Dose‑dependent decreases in the cell proliferation and differentiation were observed in dexamethasone (Dex)‑treated osteoblasts, evidenced by downregulation in the activity of alkaline phosphatasedecreased expression levels of Runt‑related transcription factor 2 and osteocalcin, and upregulated expression of RANK ligand. Dex also induced apoptosis and inhibited autophagy of osteoblasts, evidenced by upregulated B‑cell lymphoma 2 (Bcl‑2)‑associated X protein/Bcl‑2 ratio and the activation of mammalian target of rapamycin (mTOR), and decreased expression levels of Beclin‑1, autophagy protein 5 and microtubule‑associated protein 1 light chain 3 II. The effects on cell proliferation, apoptosis and autophagy induced by Dex were reversed by β‑ecdysterone in a dose‑dependent manner. Therefore, β‑ecdysterone may be a promising candidate drug for the treatment of osteoporosis through inducing osteoblast autophagic activity by inactivating mTOR.

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  • Within the in vitro studies of ecdysterone, no serious effects were reported.

    “Within these studies, no serious effects were reported [52,53,54,55,56,57,58,60,61,62,63,64,65,66,67,68,69,70,71,72,73].”

    From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [58]

    The Results (page unavailable) report that β-ecdysterone increased proliferation and reduced apoptosis in dexamethasone-treated osteoblasts. That supports an absence of serious harmful effects in the outcomes measured, but the paper does not assess or explicitly report serious adverse effects more broadly. (LLM, conf=0.76)

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  • In vitro studies associate ecdysterone with endothelial dysfunction prevention, osteoporosis prevention, glucose regulation, cytotoxic effects, antibacterial properties, immunomodulatory effects, and anti-inflammatory activities.

    “Moreover, the effects on cell lines are associated with endothelial dysfunction prevention, osteoporosis prevention, glucose regulation, cytotoxic effects, antibacterial properties, immunomodulatory effects, and anti-inflammatory activities [52,53,54,55,56,57,58,60,61,62,63,64,65,66,67,68,69,70,71,72,73].”

    From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [58]

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Cited by

Sorted by citations

Authors
Tang, Y., Yue, Z., Li, G., Zeng, L., Xin, D., Hu, Z., & Xu, C.
Affiliations
  • Department of Orthopedics, Xiaoshan TCM Hospital, Hangzhou, Zhejiang 311201, P.R. China
  • Department of Anesthesiology, Xiaoshan TCM Hospital, Hangzhou, Zhejiang 311201, P.R. China
Corresponding author
Li, Guo‑Song
Venue
Molecular Medicine Reports
Publisher
Spandidos Publications
Published
2017-10-20
Volume
17
Issue
1
Pages
158-164
ISSN
  • 1791-2997 (ISSN-L)
  • 1791-3004
DOI
10.3892/mmr.2017.7840
PMC
PMC5780097
OpenAlex
W2766251313
Cited by
36
References
5
Citations by year
2026: 2 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 5 · 2019: 4 · 2018: 2
FWCI
1.23
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Molecular Medicine Reports (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
glucocorticoid-induced osteoporosis, osteoblasts, dexamethasone, apoptosis, autophagy, mTOR signaling, Beclin-1, RANK ligand, osteogenic differentiation, alkaline phosphatase, osteocalcin, Bax/Bcl-2 ratio, mesenchymal stem cells
MeSH terms
Animals, Autophagy, Cell Differentiation, Ecdysterone, Glucocorticoids, Male, Osteoblasts, Apoptosis, Mice, Mesenchymal Stem Cells

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