Dynamic nestin expression during hair follicle maturation and the normal hair cycle
Open accessAbstract
Nestin, a type-VI intermediate filament protein, serves as a marker for neural stem cells, and is also known to be expressed in follicle stem cells. Hair follicles go through repeated cycles of anagen (growth), catagen (regression) and telogen (quiescence) throughout the life of mammals following morphogenesis. In the present study it was demonstrated that in mice, the maturation of hair follicles includes the period between morphogenesis and the first anagen (4 weeks of age). Skin samples from Nestin‑green fluorescent protein (GFP)+ mice at different hair follicle stages were collected, and immunostaining for Nestin and Ki67 was performed. It was identified that during morphogenesis, Nestin‑GFP expression was rarely detected and it gradually increased during maturation (0‑4 weeks) in hair follicle dermal cells. In mature hair follicle dermal cells, Nestin and the proliferation marker Ki67 were highly expressed in anagen, while during telogen, they were markedly decreased. Additionally, lineage tracing data demonstrated that peri‑follicular Nestin+ cells during morphogenesis differentiated into cluster of differentiation 31+ cells.
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T-SKOs resembled the natural process of HF development in vivo.
“resembled the natural process of HF development in vivo [23, 24].”
From The empowering influence of air-liquid interface culture on skin organoid hair follicle development (2025) · cites this paper as [24]
The Discussion describes the natural sequence of hair-follicle morphogenesis and postnatal cycling in mice. The paper does not examine T-SKOs or compare their development with that sequence, so it supports the in vivo reference process but not the claimed resemblance. (LLM, conf=0.78)
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Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction
Kageyama, T., Shimizu, A., Anakama, R., Nakajima, R., Suzuki, K., Okubo, Y., & Fukuda, J. (2022) · Science Advances · doi:10.1126/sciadv.add4603 · Cited by 73 · see citing works
The empowering influence of air-liquid interface culture on skin organoid hair follicle development
Sun, J., Ahmed, I., Brown, J., Khosrotehrani, K., & Shafiee, A. (2025) · Burns & Trauma · doi:10.1093/burnst/tkae070 · Cited by 19 · see citing works
- Authors
- Chen, R., Miao, Y., & Hu, Z.
- Affiliations
- Nanfang Hospital (CN)
- Venue
- Molecular Medicine Reports
- Publisher
- Spandidos Publications
- Published
- 2018-11-26
- Volume
- 19
- Issue
- 1
- Pages
- 549-554
- ISSN
- 1791-2997 (ISSN-L)
- 1791-3004
- OpenAlex
- W2903261357
- Cited by
- 7
- References
- 0
- Citations by year
- 2024: 2 · 2022: 2 · 2020: 1 · 2019: 2
- FWCI
- 1.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Medicine Reports (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (201508020262, 81701929, 81772104, 81471900)
- Natural Science Foundation of Guangdong Province (2015A030311001, 2017A030310120)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
- Keywords
- nestin, hair cycle, anagen, telogen, catagen, hair follicle morphogenesis, intermediate filaments, follicle stem cells, dermal papilla cells, Ki-67, cell proliferation, lineage tracing
- MeSH terms
- Animals, Cell Differentiation, Morphogenesis, Skin, Stem Cells, Hair Follicle, Ki-67 Antigen, Cell Proliferation, Green Fluorescent Proteins, Mice, Nestin
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