Nuclear expression of claudin‑3 in human colorectal adenocarcinoma cell lines and tissues
Open accessAbstract
Claudins are members of a large family of transmembrane proteins, which are essential for the formation of tight junctions and have a significant effect on the biological behavior of tumor progression. Previous studies have demonstrated that several claudins show aberrant expression patterns in numerous types of cancer. The present study investigated the expression and localization of claudin-3 and claudin-7 in human colorectal adenocarcinoma cell lines and tissues. The protein expression levels of claudin-3 and claudin-7 were determined using immunocytochemical and immunohistochemical staining. Claudin-3, but not claudin-7, exhibited nuclear localization in the human colorectal adenocarcinoma Caco-2 and SW620 cell lines. Surgically resected colorectal adenocarcinoma tissue specimens were obtained, and the associations between the expression of claudin-3 or claudin-7 and various clinicopathological parameters were analyzed. The membranous expression rates of claudin-3 and claudin-7 were 58.0 and 50.0%, while their nuclear expression rates were 22.0 and 2.0%, respectively. The membranous expression of claudin-3 and claudin-7 was not associated with any clinicopathological factors, whereas the nuclear expression of claudin-3 was associated with histological type and was significantly increased in colorectal mucinous adenocarcinomas compared with that in well- to moderately-differentiated colorectal adenocarcinomas (P<0.01). However, no associations were observed between the nuclear expression of claudin-7 and any clinicopathological parameter. In conclusion, the nuclear expression of claudin-3 in colorectal mucinous adenocarcinoma may be involved in the biological transformation of tumors. The results from the present study indicated that claudin-3 is an important protein associated with histological type and has potential as a prognostic marker. Although the mechanisms underlying the nuclear localization of claudin-3 in tumorigenesis have not yet been elucidated in detail, the present results indicated the potential of claudin-3 as a histopathological biomarker for colorectal adenocarcinomas.
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Claudin-3 has been shown to control malignancy and metastasis in CRC.
“Whereas claudin-3 has been shown to control malignancy and metastasis in CRC,52,53 claudin-2 led to a leaky epithelium in tumour tissue.54”
From PU.1-driven Th9 Cells Promote Colorectal Cancer in Experimental Colitis Models Through Il-6 Effects in Intestinal Epithelial Cells (2022) · cites this paper as [52]
The passage accompanying Table IV (page not supplied) reports that nuclear claudin-3 expression is higher in mucinous colorectal adenocarcinomas and advanced T4 tumors, supporting an association with tumor behavior. It explicitly says further studies are needed to establish whether claudin-3 regulates proliferation or metastasis, so it does not show that claudin-3 controls malignancy and metastasis. (LLM, conf=0.88)
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Fagotto, F., Glück, U., & Gumbiner, B. M. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70082-x · Cited by 434 · see citing works
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Research works printed but not linked: 1
- Japanese Society for Cancer of the Colon and Rectum: Japanese Classification of Colorectal Carcinoma. 8th edition, Kanehara, Tokyo, 2013.
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Gerlach, K., Popp, V., Wirtz, S., Al-Saifi, R., Gonzalez Acera, M., Atreya, R., Dregelies, T., Vieth, M., Fichtner-Feigl, S., McKenzie, A. N. J., et al. (2022) · Journal of Crohn's and Colitis · doi:10.1093/ecco-jcc/jjac097 · Cited by 25 · see citing works
- Authors
- Tokuhara, Y., Morinishi, T., Matsunaga, T., Sakai, M., Sakai, T., Ohsaki, H., Kadota, K., Kushida, Y., Haba, R., & Hirakawa, E.
- Affiliations
- Laboratory of Pathology, Department of Medical Technology, Kagawa Prefectural University of Health Sciences, Takamatsu, Kagawa 761‑0123, Japan
- Kagawa University (JP)
- Osaka Dental University Hospital (JP)
- The University of Osaka (JP)
- Kobe University (JP)
- ORCID
- Venue
- Oncology Letters
- Publisher
- Spandidos Publications
- Published
- 2017-10-26
- Volume
- 15
- Issue
- 1
- Pages
- 99-108
- ISSN
- 1792-1074 (ISSN-L)
- 1792-1082
- PMC
- PMC5738677
- OpenAlex
- W2766438229
- Cited by
- 23
- References
- 1
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2022: 3 · 2021: 6 · 2020: 3 · 2019: 3 · 2018: 2
- FWCI
- 1.09
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Oncology Letters (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (15K15737, 15K08381, 15H02575)
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- claudin-3, claudin-7, colorectal adenocarcinoma, nuclear localization, tight junction proteins, mucinous adenocarcinoma, tumor differentiation, prognostic factors, clinicopathological correlation, Caco-2 cells, SW620 cells
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