Expression of the Arp2/3 complex in human gliomas and its role in the migration and invasion of glioma cells
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A hallmark of directional cell migration is localized actin polymerization at the leading protrusions of the cell. The Arp2/3 complex nucleates the formation of the dendritic actin network (lamellipodia) at the leading edge of motile cells. This study was designed to investigate the role of the Arp2/3 complex in the infiltrative behavior of glioma cells. Immunofluorescence and western blotting showed a positive correlation between the expression of Arp2/3 and the malignancy of glioma specimens (r=0.686, P=0.02) and confocal microscopy demonstrated localization of the Arp2/3 complex in lamellipodia of glioma cells. Furthermore, we examined the effects of Arp2/3 complex inhibition in U251, LN229 and SNB19 glioma cells using CK666, an Arp2/3 complex inhibitor. Glioma cells lost lamellipodia and cell polarity after treatment with CK666. Inhibition of the Arp2/3 complex significantly affected the ability of glioma cells to migrate and invade. In the wound-healing assay, CK666 markedly inhibited cell migration, U251 cell migration was inhibited to 38.73±3.45% of control, LN229 cells to 57.40±2.16% of control and SNB19 cells to 34.17±3.82% of control. Also, CK666 significantly impaired Transwell chamber invasion capability of U251, LN229 and SNB19 cells compared with DMSO control by 72.70±4.86, 39.12±8.42 and 41.41±4.66%, respectively. The Arp2/3 complex is, therefore, likely to be a crucial participant in glioma cell invasion and migration, and may represent a target for therapeutic intervention.
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Overexpression of the ARP2/3 complex is observed in a variety of human cancers.
“Overexpression of the ARP2/3 complex has been observed in a variety of human cancers”
From SHARPIN S146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis (2022) · cites this paper as Liu, Yang et al., 2013
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SHARPIN S146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis
Butt, U., Khan, M. H., Pouwels, J., & Westermarck, J. (2022) · Journal of Cell Science · doi:10.1242/jcs.260627 · Cited by 6 · see citing works
SHARPIN serine 146 phosphorylation mediates ARP2/3 interaction, cancer cell invasion and metastasis
Butt, U., Khan, M. H., Pouwels, J., & Westermarck, J. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.01.21.477220 · Cited by 6 · see citing works
- Authors
- LIU, Z., YANG, X., CHEN, C., LIU, B., REN, B., WANG, L., ZHAO, K., YU, S., & MING, H.
- Affiliations
- Tianjin Medical University General Hospital (CN)
- Venue
- Oncology Reports
- Publisher
- Spandidos Publications
- Published
- 2013-08-20
- Volume
- 30
- Issue
- 5
- Pages
- 2127-2136
- ISSN
- 1021-335X (ISSN-L)
- 1791-2431
- OpenAlex
- W2007173580
- Cited by
- 59
- References
- 0
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 5 · 2023: 10 · 2022: 8 · 2021: 3 · 2020: 3 · 2019: 8 · 2018: 3 · 2017: 6 · 2016: 5 · 2015: 2 · 2014: 2
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Oncology Reports (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81272782)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- Arp2/3 complex, glioma, cell migration, tumor invasion, lamellipodia, actin polymerization, cell polarity, wound healing assay, glioma malignancy, therapeutic target
- MeSH terms
- Actins, Brain Neoplasms, Cell Movement, Dendritic Cells, Glioma, Humans, Neoplasm Invasiveness, Pseudopodia, Gene Expression Regulation, Neoplastic, Cell Polarity, Microscopy, Confocal, Cell Line, Tumor, Actin-Related Protein 2-3 Complex
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