Immune priming in Armadillidium vulgare against Salmonella enterica: direct or indirect costs on life history traits?

Abstract

Invertebrate immune priming is defined as an enhanced protection against secondary pathogenic infections when individuals have been previously exposed to the same or a different pathogen. Immune priming can be energetically costly for individuals, thus impacting trade-offs between life-history traits, like reproduction, growth, and lifetime. Here, the reproductive cost(s) and senescence patterns of immune priming against S. enterica in the common woodlouse A. vulgare (Crustacea, Isopoda) were investigated. Four different groups of females were used that either (1) have never been injected (control), (2) were injected twice with S. enterica (7 days between infections), (3) were firstly injected with LB-broth, then with S. enterica, and (4) females injected only once with S. enterica. All females were allowed to breed with one non-infected male and were observed for eight months. Then, the number of clutches produced, the time taken to produce the clutch(es), the number of offspring in each clutch, the senescence biomarkers of females, and parameters of their haemocytes were compared. The result was that immune priming did not significantly impact reproductive abilities, senescence patterns, and haemocyte parameters of female A. vulgare, but had an indirect effect through body weight. The lighter immune primed females took less time to produce the first clutch, which contained less offspring, but they were more likely to produce a second clutch. The opposite effects were observed in the heavier immune primed females. By highlighting that immune priming was not as costly as expected in A. vulgare, these results provide new insights into the adaptive nature of this immune process.