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Metal Cation Requirements for Phytohemagglutinin-Induced Transformation of Human Peripheral Blood Lymphocytes
Abstract
Citrate and disodium EDTA were effective inhibitors of PHA-induced lymphocyte transformation. A high concentration of citrate (24 mM) was required. Citrate inhibition was totally reversible by calcium and half as effectively by magnesium. In experiments in calcium-magnesium-free medium, calcium was determined to be five times as effective as magnesium in promoting transformation. EDTA was effective in low concentrations (1.6 mM) in blocking transformation. This effect was not entirely reversible by calcium, and was not detectably affected by magnesium. Several other metal cations were effective in reversing EDTA inhibition of PHA-induced transformation. Zinc and iron appear likely to be essential for PHA-induced lymphocyte transformation in vitro.
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Citations
Cited by
The role of calcium ions in initiating transformation of lymphocytes
MAINO, V. C., GREEN, N. M., & CRUMPTON, M. J. (1974) · Nature · doi:10.1038/251324b0 · Cited by 270
Beck, F. W. J., Kaplan, J., Fine, N., Handschu, W., & Prasad, A. S. (1997) · Journal of Laboratory and Clinical Medicine · doi:10.1016/s0022-2143(97)90091-3 · Cited by 79
Metadata
- Authors
- Alford, R. H.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1970-03-01
- Volume
- 104
- Issue
- 3
- Pages
- 698-703
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1538523410
- Cited by
- 185
- References
- 0
- FWCI
- 4.27
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Metal Cation Requirements for Phytohemagglutinin-Induced Transformation of Human Peripheral Blood Lymphocytes. https://app.cochat.ai/papers/10.4049/jimmunol.104.3.698
- The paper
- Alford, R. H. (1970). Metal Cation Requirements for Phytohemagglutinin-Induced Transformation of Human Peripheral Blood Lymphocytes. The Journal of Immunology. https://doi.org/10.4049/jimmunol.104.3.698
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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