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Constitutive production of a unique lymphokine (IL 3) by the WEHI-3 cell line.
Abstract
It has been recently demonstrated that conditioned media from Con A-stimulated splenocyte cultures contain a novel lymphokine termed IL 3. IL 3 is characterized by its ability to induce 20-alpha-hydroxysteroid dehydrogenase in spleen cells from young nu/nu mice. In search of a convenient source for biochemical and in vivo studies of this lymphokine, a number of established murine cell lines were screened for the constitutive and induced production of Il 3. It was found that WEHI-3 cells, originally designated as a myelomonocyte cell line, produced high levels of IL 3 constitutively. Added mitogen and/or phorbol acetate did not enhance the production of IL 3. Production of IL 3 varied among various sublines of WEHI-3. IL 3 purified from WEHI-3-conditioned media has biochemical and biologic characteristics identical to those obtained from Con A-conditioned media. WEHI-3 conditioned media, however, generally contained 100-fold higher levels of IL 3 than conditioned media from Con A-stimulated splenic lymphocytes.
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Citations
Cited by
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Daley, G. Q., Van Etten, R. A., & Baltimore, D. (1990) · Science · doi:10.1126/science.2406902 · Cited by 1,756
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Giulian, D., & Baker, T. (1986) · The Journal of Neuroscience · doi:10.1523/jneurosci.06-08-02163.1986 · Cited by 1,313
Razin, E., Ihle, J. N., Seldin, D., Mencia-Huerta, J. M., Katz, H. R., LeBlanc, P. A., Hein, A., Caulfield, J. P., Austen, K. F., & Stevens, R. L. (1984) · The Journal of Immunology · doi:10.4049/jimmunol.132.3.1479 · Cited by 379
Self-citationPierce, J. H., Paolo Di Fiore, P., Aaronson, S. A., Potter, M., Pumphrey, J., Scott, A., & Ihle, J. N. (1985) · Cell · doi:10.1016/s0092-8674(85)80049-0 · Cited by 320
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Metadata
- Authors
- Lee, J. C., Hapel, A. J., & Ihle, J. N.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1982-06-01
- Volume
- 128
- Issue
- 6
- Pages
- 2393-2398
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2122228460
- Cited by
- 222
- References
- 0
- FWCI
- 5.12
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lee, J. C., Hapel, A. J., & Ihle, J. N. (1982). Constitutive production of a unique lymphokine (IL 3) by the WEHI-3 cell line. The Journal of Immunology. https://doi.org/10.4049/jimmunol.128.6.2393
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
