The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Bioactive IL-18 Expression Is Up-Regulated in Crohn’s Disease
Open accessAbstract
An imbalance of immunoregulatory factors is believed to contribute to uncontrolled mucosal Th1 cell activation in Crohn’s disease (CD). IL-18, a macrophage-like cell-derived cytokine, is involved in Th1 clone development, and IFN-γ production. Therefore, IL-18 expression was investigated in CD. Whole mucosal intestinal tissue and lamina propria mononuclear cells (LPMC) of 12 CD and 9 ulcerative colitis (UC) patients and 15 non-inflammatory bowel disease (IBD) controls were tested for IL-18 by semiquantitative RT-PCR and Western blot analysis. Transcripts for IL-18 were found in all samples tested. However, increased IL-18 mRNA accumulation was detected in both mucosal and LPMC samples from CD in comparison to UC and controls. In CD, transcripts for IL-18 were more abundant in the mucosal samples taken from involved areas. An 18-kDa band consistent with mature IL-18 was predominantly found in CD mucosal samples. In mucosal samples from non-IBD controls, IL-18 was present as a 24-kDa polypeptide. Consistently, active IL-1β-converting enzyme (ICE) subunit (p20) was expressed in samples from either CD or UC, whereas, in colonic mucosa from non-IBD controls, ICE was synthesized as precursor (p45) only. To confirm that IL-18 produced in CD tissue was functionally active, CD LPMC were treated with a specific IL-18 antisense oligonucleotide. In these cultures, IL-18 down-regulation was accompanied by a decrease in IFN-γ expression. In aggregate, our data indicate that IL-18 up-regulation is a feature of CD and suggest that IL-18 may contribute to the local immunoinflammatory response in CD.
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Cited by
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Interleukin-18 Regulates Both Th1 and Th2 Responses
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Metadata
- Authors
- Monteleone, G., Trapasso, F., Parrello, T., Biancone, L., Stella, A., Iuliano, R., Luzza, F., Fusco, A., & Pallone, F.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-07-01
- Volume
- 163
- Issue
- 1
- Pages
- 143-147
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1501206772
- Cited by
- 398
- References
- 28
- FWCI
- 17.39
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Monteleone, G., Trapasso, F., Parrello, T., Biancone, L., Stella, A., Iuliano, R., Luzza, F., Fusco, A., & Pallone, F. (1999). Bioactive IL-18 Expression Is Up-Regulated in Crohn’s Disease. The Journal of Immunology. https://doi.org/10.4049/jimmunol.163.1.143
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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