The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Adenosine 5′-Monophosphate-Activated Protein Kinase Promotes Macrophage Polarization to an Anti-Inflammatory Functional Phenotype
Open accessAbstract
Herein, we demonstrate a role of AMP-activated protein kinase (AMPK) as a potent counterregulator of inflammatory signaling pathways in macrophages. Stimulation of macrophages with anti-inflammatory cytokines (i.e., IL-10 and TGFβ) resulted in the rapid phosphorylation/activation of AMPK, whereas stimulation of macrophages with a proinflammatory stimulus (LPS) resulted in AMPK dephosphorylation/inactivation. Inhibition of AMPKα expression by RNA interference dramatically increased the mRNA levels of LPS-induced TNF-α, IL-6, and cyclooxygenase-2. Likewise, expression of a dominant negative AMPKα1 in macrophages enhanced TNF-α and IL-6 protein synthesis in response to LPS stimulation, while diminishing the production of IL-10. In contrast, transfection of macrophages with a constitutively active form of AMPKα1 resulted in decreased LPS-induced TNF-α and IL-6 production, and heightened production of IL-10. In addition, we found that AMPK negatively regulated LPS-induced IκB-α degradation and positively regulated Akt activation, accompanied by inhibition of glycogen synthase kinase β and activation of CREB. Thus, AMPK directs signaling pathways in macrophages in a manner that suppresses proinflammatory responses and promotes macrophage polarization to an anti-inflammatory functional phenotype.
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Cites
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Self-citationMacrophage Polarization Comes of Age
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“The designations M1 and M2 have also been used to categorize macrophages expressing a proinflammatory vs an anti-inflammatory functional profile, respectively (34, 35).”
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Cited by
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Anti-inflammatory effect of IL-10 mediated by metabolic reprogramming of macrophages
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Mitochondria are the powerhouses of immunity
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Metadata
- Authors
- Sag, D., Carling, D., Stout, R. D., & Suttles, J.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-12
- Volume
- 181
- Issue
- 12
- Pages
- 8633-8641
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W2129224427
- Cited by
- 796
- References
- 12
- FWCI
- 7.03
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Sag, D., Carling, D., Stout, R. D., & Suttles, J. (2008). Adenosine 5′-Monophosphate-Activated Protein Kinase Promotes Macrophage Polarization to an Anti-Inflammatory Functional Phenotype. The Journal of Immunology. https://doi.org/10.4049/jimmunol.181.12.8633
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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