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Cited by 35Toxoplasma gondii Research StudiesCoccidia and coccidiosis researchParasitic infections in humans and animals

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Toxoplasma gondii tkl1 Deletion Mutant Is a Promising Vaccine against Acute, Chronic, and Congenital Toxoplasmosis in Mice

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Wang, Jin-Lei, Liang, Qin-Li, Li, Ting-Ting, He, Jun-Jun, Bai, Meng-Jie, Cao, Xue-Zhen, Elsheikha, Hany M, Zhu, Xing-Quan·2020·The Journal of Immunology
DOI 10.4049/jimmunol.1900410
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Abstract

In this study, we generated a tkl1 deletion mutant in the Toxoplasma gondii type 1 RH (RHΔtkl1) strain and tested the protective efficacies of vaccination using RHΔtkl1 tachyzoites against acute, chronic, and congenital T. gondii infections in Kunming mice. Mice vaccinated with RHΔtkl1 mounted a strong humoral and cellular response as shown by elevated levels of anti–T. gondii–specific IgG, IL-2, IL-12, IFN-γ, and IL-10. All RHΔtkl1-vaccinated mice survived a lethal challenge with 1 × 103 tachyzoites of type 1 RH or ToxoDB#9 (PYS or TgC7) strain as well as 100 cysts or oocysts of Prugniuad strain. All mock-vaccinated plus infected mice have died. Vaccination also protected against cyst- or oocyst-caused chronic infection, reduced vertical transmission caused by oocysts, increased litter size, and maintained body weight of pups born to dams challenged with 10 oocysts on day 5 of gestation. In contrast, all mock-vaccinated plus oocysts-infected dams had aborted, and no fetus has survived. Vaccinated dams remained healthy postinfection, and their brain cyst burden was significantly reduced compared with mock-vaccinated dams infected with oocysts. In vivo depletion of CD4+ T cells, CD8+ T cells, and B cells revealed that CD8+ T cells are involved in the protection of mice against T. gondii infection. Additionally, adoptive transfer of CD8+ T cells from RHΔtkl1-vaccinated mice significantly enhanced the survival of naive mice infected with the pathogenic strain. Together, these data reaffirm the importance of CD8+ T cell responses in future vaccine design for toxoplasmosis and present T. gondii tkl1 gene as a promising vaccine candidate.

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Metadata

Authors
Wang, J.-L., Liang, Q.-L., Li, T.-T., He, J.-J., Bai, M.-J., Cao, X.-Z., Elsheikha, H. M., & Zhu, X.-Q.
Affiliations
  • Lanzhou Veterinary Research Institute (CN)
  • State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou, Gansu Province 730046 ,
  • University of Nottingham (GB)
Corresponding author
He, Jun-Jun
ORCID
Venue
The Journal of Immunology
Publisher
Oxford University Press (OUP)
Published
2020-03
Volume
204
Issue
6
Pages
1562-1570
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
DOI
10.4049/jimmunol.1900410
OpenAlex
W3003301494
Cited by
35
References
0
Citations by year
2026: 4 · 2025: 4 · 2024: 9 · 2023: 6 · 2022: 4 · 2021: 3 · 2020: 5
FWCI
3.38
Type
journal article
Language
en
Open access
Licence
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • Repository@Nottingham (University of Nottingham) (acceptedVersion)
  • Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Toxoplasma gondii Research Studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Coccidia and coccidiosis research
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasitic infections in humans and animals
Keywords
Toxoplasma gondii, toxoplasmosis vaccine, congenital toxoplasmosis, chronic toxoplasmosis, acute toxoplasmosis, vertical transmission, CD8+ T cells, adoptive T cell therapy, humoral and cellular immune response, brain cyst burden, oocysts, vaccine candidate
MeSH terms
Acute Disease, Animals, Chronic Disease, Disease Models, Animal, Female, Humans, Male, Toxoplasma, Toxoplasmosis, Animal, Toxoplasmosis, Congenital, Virulence, Protozoan Proteins, Protozoan Vaccines, Genes, Protozoan, Sequence Deletion, CD8-Positive T-Lymphocytes, Infectious Disease Transmission, Vertical, Adoptive Transfer, Virulence Factors, Mice, Livestock

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CoChat. (2026). Citation check: Toxoplasma gondii tkl1 Deletion Mutant Is a Promising Vaccine against Acute, Chronic, and Congenital Toxoplasmosis in Mice. https://app.cochat.ai/papers/10.4049/jimmunol.1900410
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Wang, J.-L., Liang, Q.-L., Li, T.-T., He, J.-J., Bai, M.-J., Cao, X.-Z., Elsheikha, H. M., & Zhu, X.-Q. (2020). Toxoplasma gondii tkl1 Deletion Mutant Is a Promising Vaccine against Acute, Chronic, and Congenital Toxoplasmosis in Mice. The Journal of Immunology. https://doi.org/10.4049/jimmunol.1900410