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Dengue Virus Identification by the Plaque Reduction Neutralization Test
Abstract
Summary: A method for identification and serologic classification of dengue viruses using a plaque reduction neutralization test and monkey immune serum is described. Results of typing 40 strains confirm the usefulness of this method for classification of low passage strains. Adaptation of the isolates to suckling mice or tissue culture was not required and strains within each of the four dengue serotypes were readily identified. Cross neutralization tests using monkey antiserum confirmed the results of the one-way neutralization test using antiserum to reference dengue strains and also indicated antigenic variation within serotypes 1, 2 and 4.
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Citations
Cited by
Dengue: a continuing global threat
Guzman, M. G., Halstead, S. B., Artsob, H., Buchy, P., Farrar, J., Gubler, D. J., Hunsperger, E., Kroeger, A., Margolis, H. S., Martínez, E., et al. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2460 · Cited by 2,082
Pathogenesis of dengue: challenges to molecular biology
Halstead, S. (1988) · Science · doi:10.1126/science.239.4839.476 · Cited by 1,614
Dengue and dengue haemorrhagic fever
Rigau-Pérez, J. G., Clark, G. G., Gubler, D. J., Reiter, P., Sanders, E. J., & Vance Vorndam, A. (1998) · The Lancet · doi:10.1016/s0140-6736(97)12483-7 · Cited by 1,051
Effect of Temperature on the Vector Efficiency of Aedes aegypti for Dengue 2 Virus
Watts, D. M., Burke, D. S., Harrison, B. A., Whitmire, R. E., & Nisalak, A. (1987) · The American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1987.36.143 · Cited by 759
Self-citationHenchal, E. A., & Putnak, J. R. (1990) · Clinical Microbiology Reviews · doi:10.1128/cmr.3.4.376 · Cited by 620
Metadata
- Authors
- Russell, P. K., & Nisalak, A.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1967-08-01
- Volume
- 99
- Issue
- 2
- Pages
- 291-296
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1521072280
- Cited by
- 189
- References
- 0
- FWCI
- 6.02
- Type
- journal article
- Language
- en
- Open access
- closed
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- Russell, P. K., & Nisalak, A. (1967). Dengue Virus Identification by the Plaque Reduction Neutralization Test. The Journal of Immunology. https://doi.org/10.4049/jimmunol.99.2.291
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.