Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth
Open accessAbstract
Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulate and facilitate plant growth.The present work deals with isolation, characterization and identification of indole acetic acid producing bacteria from the rhizospheric soil.Out of ten Indole acetic acid producing isolates, five were selected as efficient producers.Optimization of indole acetic acid production was carried out at different cultural conditions of pH and temperature with varying media components such as carbon and nitrogen source, tryptophan concentration.Partial purification of IAA was done and purity was confirmed with Thin layer chromatography.Subsequently, effect on plant growth was tested by pot assay.In conclusion the study suggests the IAA producing bacteria as efficient biofertilizer inoculants to promote plant growth.
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“Accurately 4 mL of Salkowski reagent comprising 0.5 M ferric chloride (FeCl 3 ) and 35% perchloric acid (HClO 4 ) was added to the supernatant as described (Mohite, 2013).”
Influential
- Authors
- Mohite, B.
- Corresponding author
- Mohite, B
- ORCID
- Mohite, B
- Venue
- Journal of soil science and plant nutrition
- Published
- 2013
- Issue
- ahead
- Pages
- 0-0
- ISSN
- 0718-9508 (ISSN-L)
- 0718-9516
- OpenAlex
- W2156909871
- Cited by
- 501
- References
- 0
- Citations by year
- 2026: 13 · 2025: 37 · 2024: 44 · 2023: 62 · 2022: 65 · 2021: 68 · 2020: 68 · 2019: 45 · 2018: 30 · 2017: 33 · 2016: 17 · 2015: 12 · 2014: 6
- FWCI
- 14.09
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Journal of soil science and plant nutrition (publishedVersion, cc-by-nc)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, other-oa)
- Funders
- University Grants Commission
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Legume Nitrogen Fixing Symbiosis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Agriculture, Plant Science, Crop Management
- Keywords
- indole acetic acid, rhizosphere bacteria, plant growth promotion, bacterial isolation and identification, tryptophan, carbon and nitrogen sources, pH and temperature effects
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