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Effect of Helix aspersa extract on TNFα, NF-κB and some tumor suppressor genes in breast cancer cell line Hs578T

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El Ouar, Ibtissem, Braicu, Cornelia, Naimi, Dalila, Irimie, Alexendru, Berindan-Neagoe, Ioana·2017·Pharmacognosy Magazine
DOI 10.4103/0973-1296.204618PMC PMC5421427
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Abstract

BACKGROUND: , is a big land snail widely found in the Mediterranean countries. It is one of the most consumed species and widely used in zootherapy. OBJECTIVE: . MATERIALS AND METHODS: extract was studied on a triple negative breast cancer cell line Hs578T. Firstly, the morphological changes and the mode of cell death induced by the extract have been evaluated by microscopy and acridine orange/ethidium bromide staining. The effect of the extract at dilution 0.1% and 1% was then tested on some genes, regulators of cell death and proliferation like tumor necrosis factor α (TNFα), NF- κB, and the tumor suppressor genes P53 and PTEN. RESULTS: Data demonstrate that the extract induces necrosis in tumor cells. It enhances significantly the expression of TNFα; mRNA levels were 20 and 10 times more important in treated cells compared to nontreated cells. NF-κB and PTEN were inhibited with the dilution 1% after 8 and 24 hours of treatment. P53 expression was further inhibited but only with the highest dose, after 4, 8, and 24 hours. CONCLUSION: AO: acridine orange; Bcl-2: B cell lymphoma 2. cDNA: complementary DNA; ELISA: enzyme linked immunosorbent assay; EB: ethidium bromide; IC50: the half maximal inhibitory concentration; mRNA: messenger RNA. MAPK: mitogen-activated protein kinase; NF-κB: nuclearfactorkappa B; PBS: phosphate buffered saline. PI3K: phospho-inositol 3 kinase; PTEN: phosphatase and tensin homolog; ROS: reactive oxygen species. RT-PCR: reverse transcription polymerase chain reaction; TNFα: tumor necrosis factor alpha. TNFR1: TNF receptor-1; TP53: tumor protein 53.

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Cited by

Sorted by citations

Authors
El Ouar, I., Braicu, C., Naimi, D., Irimie, A., & Berindan-Neagoe, I.
Affiliations
Iuliu Hațieganu University of Medicine and Pharmacy (RO)
ORCID
Braicu, Cornelia
Venue
Pharmacognosy Magazine
Publisher
SAGE Publications
Published
2017
Volume
13
Issue
50
Pages
281
ISSN
0973-1296 (ISSN-L)
DOI
10.4103/0973-1296.204618
PMC
PMC5421427
OpenAlex
W2606646434
Cited by
24
References
0
Citations by year
2026: 2 · 2025: 6 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 3 · 2018: 2
FWCI
1.27
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Pharmacognosy Magazine (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Mollusks and Parasites Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
Keywords
Helix aspersa, triple-negative breast cancer, Hs578T cells, TNF-α, NF-κB, p53, PTEN, tumor suppressor, necrosis, cell death, zootherapy, anticancer activity

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