Spermiogram and sperm head morphometry assessed by multivariate cluster analysis results during adolescence (12-18 years) and the effect of varicocele
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This work evaluates sperm head morphometric characteristics in adolescents from 12 to 18 years of age, and the effect of varicocele. Volunteers between 150 and 224 months of age (mean 191, n = 87), who had reached oigarche by 12 years old, were recruited in the area of Barranquilla, Colombia. Morphometric analysis of sperm heads was performed with principal component (PC) and discriminant analysis. Combining seminal fluid and sperm parameters provided five PCs: two related to sperm morphometry, one to sperm motility, and two to seminal fluid components. Discriminant analysis on the morphometric results of varicocele and nonvaricocele groups did not provide a useful classification matrix. Of the semen-related PCs, the most explanatory (40%) was related to sperm motility. Two PCs, including sperm head elongation and size, were sufficient to evaluate sperm morphometric characteristics. Most of the morphometric variables were correlated with age, with an increase in size and decrease in the elongation of the sperm head. For head size, the entire sperm population could be divided into two morphometric subpopulations, SP1 and SP2, which did not change during adolescence. In general, for varicocele individuals, SP1 had larger and more elongated sperm heads than SP2, which had smaller and more elongated heads than in nonvaricocele men. In summary, sperm head morphometry assessed by CASA-Morph and multivariate cluster analysis provides a better comprehension of the ejaculate structure and possibly sperm function. Morphometric analysis provides much more information than data obtained from conventional semen analysis.
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Some abnormalities related to varicocele include elongated sperm head and abnormal retention of cytoplasmic droplets.
“Some abnormalities that are related to varicocele include elongated sperm head and abnormal retention of cytoplasmic droplets (68,69).”
From Isolated teratozoospermia: revisiting its relevance in male infertility: a narrative review (2024) · cites this paper as [68]
The Results (Sperm head morphometric subpopulations; page unavailable) report more elongated sperm heads in subpopulations from adolescents with varicocele. The paper does not report abnormal retention of cytoplasmic droplets, so it supports only the sperm-head portion of the claim. (LLM, conf=0.88)
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- Authors
- Soler, C., Vásquez, F., Camps, P., Valverde, A., & García-Molina, A.
- Affiliations
- Universitat de València (ES)
- Universidad del Norte (CO)
- Corresponding author
- Soler, Carles
- Venue
- Asian Journal of Andrology
- Published
- 2016
- Volume
- 18
- Issue
- 6
- Pages
- 824
- ISSN
- 1008-682X (ISSN-L)
- PMC
- PMC5109870
- OpenAlex
- W2538354594
- Cited by
- 26
- References
- 34
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 7 · 2017: 1 · 2016: 2
- FWCI
- 6.20
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-sa
- Locations
- Asian Journal of Andrology (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Americanae (AECID Library) (submittedVersion, cc-by-nc-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- TEC repository (Technological Institute of Costa Rica) (submittedVersion, cc-by-nc-sa)
- Funders
- Universidad del Norte
- Ministerio de Ciencia Tecnología y Telecomunicaciones
- Consejo Nacional para Investigaciones Científicas y Tecnológicas
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Health Professions › General Health Professions › Male Reproductive Health Studies
- Health Sciences › Medicine › Rheumatology › Urologic and reproductive health conditions
- Keywords
- sperm head morphometry, varicocele, sperm motility, sperm head size, semen composition, sperm subpopulations
- MeSH terms
- Adolescent, Child, Humans, Male, Sperm Head, Sperm Motility, Spermatozoa, Varicocele, Multivariate Analysis, Cluster Analysis, Cell Shape, Semen Analysis
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