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Cited by 87Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchHydrogels: synthesis, properties, applications

Contribution of Physical Methods in Decellularization of Animal Tissues

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Rabbani, Mohsen, Zakian, Nasrin, Alimoradi, Nima·2021·Journal of Medical Signals & Sensors
DOI 10.4103/jmss.jmss_2_20PMC PMC8043117
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Abstract

Biologic scaffolds composed of extracellular matrix (ECM) are frequently used for clinical purposes of tissue regeneration. Different methods have been developed for this purpose. All methods of decellularization including chemical and physical approaches leave some damage on the ECM; however, the effects of these methods are different which make some of these procedures more proper to maintain ECM structure than other methods. This review is aimed to introduce and compare new physical methods for the decellularization of different tissues and organs in tissue engineering. All recent reports and research that have used at least one physical method in the procedure of decellularization, were included and evaluated in this paper. The advantages and drawbacks of each method were examined and compared considering the effectiveness. This review tried to highlight the prospective potentials and benefits of applying physical methods for decellularization protocols in tissue engineering instead of the current chemical methods. These chemical methods are harsh in nature and were shown to be destructive and harmful to essential substances of ECM and scaffold structure. Therefore, using physical methods as a partial or even a whole protocol could save time, costs, and quality of the final acellular tissue in complicated decellularization procedures. Moreover, regarding the control factor that could be achieved easily with physical methods, optimization of different decellularization protocols would be quite satisfactory. Combined methods take advantage of both chemical and physical approaches.

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  • Physical decellularization techniques' less toxicity makes them attractive options for adjuvant treatment for enhancing chemical and biological agent effectiveness.

    “Nonetheless, their less toxicity makes them attractive options for adjuvant treatment for enhancing the chemical and biological agent effectiveness [67].”

    From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [67]

    The Abstract and Combined methods section (pages not provided) describe physical methods as less damaging to tissue structure and say that combining methods improves decellularization efficiency. The Introduction calls chemical agents toxic, but the paper does not establish that physical methods have lower toxicity or that lower toxicity is what makes them useful alongside chemical and enzymatic agents. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • Vacuum-assisted decellularization, employing negative pressure to expedite the process, can be utilized in conjunction with other techniques to enhance outcomes.

    “Vacuum‐assisted decellularization, employing negative pressure to expedite the process, can be utilized in conjunction with other techniques to enhance outcomes.”

    From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [83]

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References 65 · Cited by 87

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Authors
Rabbani, M., Zakian, N., & Alimoradi, N.
Affiliations
University of Isfahan (IR)
Corresponding author
Rabbani, Mohsen
ORCID
Alimoradi, Nima
Venue
Journal of Medical Signals & Sensors
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2021-01
Volume
11
Issue
1
Pages
1-11
ISSN
2228-7477 (ISSN-L)
DOI
10.4103/jmss.jmss_2_20
PMC
PMC8043117
OpenAlex
W3126606565
Cited by
87
References
65
Citations by year
2026: 11 · 2025: 22 · 2024: 25 · 2023: 14 · 2022: 13 · 2021: 2
FWCI
3.25
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • Journal of Medical Signals & Sensors (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
decellularization, extracellular matrix, biological scaffolds, tissue engineering, tissue regeneration

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