DNA extraction and polymerase chain reaction
Open accessAbstract
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient quantity. DNA can be amplified with the help of PCR. The basic principle and different variants of PCR are discussed.
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Non-organic techniques for PDRN extraction use milder conditions, such as neutral lysis buffers and shorter reaction times.
“In contrast, non-organic techniques use milder conditions, such as neutral lysis buffers and shorter reaction times”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [162]
The DNA EXTRACTION section identifies nonorganic DNA extraction methods, and the Organic extraction section calls the organic method time-consuming. Neither section describes PDRN extraction or says that nonorganic methods use neutral lysis buffers or shorter reaction times. (LLM, conf=0.82)
Experimental — accuracy not yet measuredNon-organic techniques for PDRN extraction show improved extraction efficiency and product purity compared with chemical methods.
“and thereby show improved extraction efficiency and product purity [162]”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [162]
The DNA EXTRACTION section (page not provided) identifies nonorganic extraction and says DNA isolation should yield sufficient, pure DNA. It does not discuss PDRN or report that nonorganic techniques improve extraction efficiency or purity compared with chemical methods. (LLM, conf=0.84)
Experimental — accuracy not yet measured
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Research works printed but not linked: 4
- [1]Lo AC Feldman SR Polymerase chain reaction: Basic concepts and clinical applications in dermatology J Am Acad Dermatol 1994 30 250 60 8288785
- [2]Clark DP Pazdernik NJ Molecular Biology, Polymerase Chain Reaction 2013 2nd ed United States of America (USA) Elsevier BV 163 93 Chap. 6
- [3]Niemz A Ferguson TM Boyle DS Point-of-care nucleic acid testing for infectious diseases Trends Biotech 2011 29 240 50 PMC3746968 21377748
- [4]Lorenz TC Polymerase chain reaction: Basic protocol plus troubleshooting and optimizing strategies J Vis Exp 2012 63 e3998 PMC4846334 22664923
Cited by
Sorted by citations
Kim, S. T. (2025) · Pharmaceutics · doi:10.3390/pharmaceutics17081024 · Cited by 18 · see citing works
- Authors
- Gupta, N.
- Affiliations
- Post Graduate Institute of Medical Education and Research (IN)
- Corresponding author
- Gupta, Nalini
- ORCID
- Gupta, Nalini
- Venue
- Journal of Cytology
- Published
- 2019
- Volume
- 36
- Issue
- 2
- Pages
- 116
- ISSN
- 0970-9371 (ISSN-L)
- PMC
- PMC6425773
- OpenAlex
- W2911651954
- Cited by
- 146
- References
- 0
- Citations by year
- 2026: 13 · 2025: 32 · 2024: 44 · 2023: 29 · 2022: 15 · 2021: 10 · 2020: 2 · 2019: 1
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by-nc-sa
- Locations
- Journal of Cytology (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- DNA extraction, PCR, DNA amplification, DNA quality and quantity
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