Inhibition by miR-410 facilitates direct retinal pigment epithelium differentiation of umbilical cord blood-derived mesenchymal stem cells
Open accessAbstract
Retinal pigment epithelium (RPE) is a major component of the eye. This highly specialized cell type facilitates maintenance of the visual system. Because RPE loss induces an irreversible visual impairment, RPE generation techniques have recently been investigated as a potential therapeutic approach to RPE degeneration. A microRNA-based technique is a new strategy for producing RPE cells from adult stem cell sources. Previously, we identified that antisense microRNA-410 (anti-miR-410) induces RPE differentiation from amniotic epithelial stem cells. In this study, we investigated RPE differentiation from umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) via anti-miR-410 treatment. We identified miR-410 as a RPE-relevant microRNA in UCB-MSCs from among 21 putative human RPE-depleted microRNAs. Inhibition of miR-410 induces overexpression of immature and mature RPE-specific factors, including MITF, LRAT, RPE65, Bestrophin, and EMMPRIN. The RPE-induced cells were able to phagocytize microbeads. Results of our microRNA-based strategy demonstrated proof-of-principle for RPE differentiation in UCB-MSCs by using anti-miR-410 treatment without the use of additional factors or exogenous transduction.
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Inhibition of miR-410 stimulates differentiation of RPE-like cells that perform efficient phagocytosis from umbilical cord blood-derived mesenchymal stem cells.
“Choi et al., based on in silico studies, showed that inhibition of miR-410 stimulates differentiation of RPE-like cells that perform efficient phagocytosis from umbilical cord blood-derived mesenchymal stem cells [274].”
From MicroRNAs as Regulators of Phagocytosis (2022) · cites this paper as [274]
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MicroRNAs as Regulators of Phagocytosis
Gierlikowski, W., & Gierlikowska, B. (2022) · Cells · doi:10.3390/cells11091380 · Cited by 25 · see citing works
- Authors
- Choi, S. W., Kim, J.-J., Seo, M.-S., Park, S.-B., Shin, T.-H., Shin, J.-H., Seo, Y., Kim, H.-S., & Kang, K.-S.
- Affiliations
- Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
- Seoul National University (KR)
- Venue
- Journal of Veterinary Science
- Publisher
- XMLink
- Published
- 2017
- Volume
- 18
- Issue
- 1
- Pages
- 59
- ISSN
- 1229-845X (ISSN-L)
- 1976-555X
- PMC
- PMC5366303
- OpenAlex
- W2433838219
- Cited by
- 21
- References
- 1
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 3
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Veterinary Science (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Journal of Veterinary Science (cc-by-nc-sa)
- Funders
- Korea Health Industry Development Institute (HI14C3301)
- Institute for Veterinary Science, Seoul National University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- miR-410, retinal pigment epithelium differentiation, human umbilical cord mesenchymal stem cells, MITF, RPE65, bestrophin, EMMPRIN, LRAT, phagocytosis, RPE degeneration, stem cell therapy
- MeSH terms
- Retinoid Isomerohydrolase, Cell Differentiation, Fetal Blood, Humans, Phagocytosis, Gene Expression Regulation, Developmental, cis-trans-Isomerases, MicroRNAs, Otx Transcription Factors, Retinal Pigment Epithelium, Mesenchymal Stem Cells
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