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Split-Marker Recombination for Efficient Targeted Deletion of Fungal Genes
Open accessAbstract
A commonly used method for fungal gene deletion is introduction of linear DNA consisting of a selectable marker gene flanked on both sides by short stretches of DNA that target a gene of interest (Wirsel et al 1996 Curr. Genet 29:241-249). Gene deletion in Cochliobolus heterostrophus and Gibberella zeae occurs efficiently with this approach. To facilitate deletion construct synthesis, we have applied the "split-marker” deletion strategy previously developed for Saccharomyces cerevisiae (Fairhead et al. 1996 Yeast 12:1439-57; Fairhead et al. 1998 Gene 223:33-46). Here, we describe both fusion PCR-based and plasmid-based deletion methods using this strategy with PEG-mediated protoplast transformation (Turgeon et al, 1985 Mol. Gen. Genet. 201:450-453). These methods are predicted to work well with any transformable fungus that undergoes homologous recombination between chromosomal and introduced DNA sequences.
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Cited by
Colot, H. V., Park, G., Turner, G. E., Ringelberg, C., Crew, C. M., Litvinkova, L., Weiss, R. L., Borkovich, K. A., & Dunlap, J. C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601456103 · Cited by 1,117
Fusarium oxysporum : exploring the molecular arsenal of a vascular wilt fungus
Pietro, A. D., Madrid, M. P., Caracuel, Z., Delgado‐Jarana, J., & Roncero, M. I. G. (2003) · Molecular Plant Pathology · doi:10.1046/j.1364-3703.2003.00180.x · Cited by 464
Oide, S., Moeder, W., Krasnoff, S., Gibson, D., Haas, H., Yoshioka, K., & Turgeon, B. G. (2006) · The Plant Cell · doi:10.1105/tpc.106.045633 · Cited by 351
Self-citationKershaw, M. J., & Talbot, N. J. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0901477106 · Cited by 317
Liu, Z., Zhang, Z., Faris, J. D., Oliver, R. P., Syme, R., McDonald, M. C., McDonald, B. A., Solomon, P. S., Lu, S., Shelver, W. L., et al. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002467 · Cited by 269
Metadata
- Authors
- Catlett, N. L., Lee, B.-N., Yoder, O. C., & Turgeon, B. G.
- Venue
- Fungal Genetics Reports
- Publisher
- New Prairie Press
- Published
- 2003-12-01
- Volume
- 50
- Issue
- 1
- Pages
- 9-11
- ISSN
- 1941-4757 (ISSN-L)
- 1941-4765
- OpenAlex
- W2593492412
- Cited by
- 488
- References
- 0
- FWCI
- 2.04
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Catlett, N. L., Lee, B.-N., Yoder, O. C., & Turgeon, B. G. (2003). Split-Marker Recombination for Efficient Targeted Deletion of Fungal Genes. Fungal Genetics Reports. https://doi.org/10.4148/1941-4765.1150
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