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Determining Atg protein stoichiometry at the phagophore assembly site by fluorescence microscopy
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“The use of imaging cytometry allows rapid and quantitative measures of the number of LC3 puncta and their relative number in individual or mixed cell types, using computerized assessment, enumeration, and data display (e.g., see refs.[42,400]).”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [42]
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Research works printed but not linked: 10
- [1]Wu JQ Pollard TD Counting cytokinesis proteins globally and locally in fission yeast Science 2005 310 310 4 16224022
- [2]Geng J Baba M Nair U Klionsky DJ Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy J Cell Biol 2008 182 129 40 18625846 PMC2447896
- [3]Kim J Huang WP Stromhaug PE Klionsky DJ Convergence of multiple autophagy and cytoplasm to vacuole targeting components to a perivacuolar membrane compartment prior to de novo vesicle formation J Biol Chem 2002 277 763 73 11675395 PMC2754695
- [4]Suzuki K Kirisako T Kamada Y Mizushima N Noda T Ohsumi Y The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation EMBO J 2001 20 5971 81 11689437 PMC125692
- [5]Xie Z Nair U Klionsky DJ Atg8 controls phagophore expansion during autophagosome formation Mol Biol Cell 2008 19 3290 8 18508918 PMC2488302
- [6]He C Song H Yorimitsu T Monastyrska I Yen WL Legakis JE Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast J Cell Biol 2006 175 925 35 17178909 PMC2064702
- [7]Yen WL Legakis JE Nair U Klionsky DJ Atg27 is required for autophagy-dependent cycling of Atg9 Mol Biol Cell 2007 18 581 93 17135291 PMC1783788
- [8]Cheong H Klionsky DJ Biochemical methods to monitor autophagy-related processes in yeast Meth Enzymol 2008 451 1 26 19185709
- [9]Ghaemmaghami S Huh WK Bower K Howson RW Belle A Dephoure N Global analysis of protein expression in yeast Nature 2003 425 737 41 14562106
- [10]Longtine MS McKenzie A III Demarini DJ Shah NG Wach A Brachat A Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae Yeast 1998 14 953 61 9717241
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Sorted by citations
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy
Klionsky, D. J., Abdalla, F. C., Abeliovich, H., Abraham, R. T., Acevedo-Arozena, A., Adeli, K., Agholme, L., Agnello, M., Agostinis, P., Aguirre-Ghiso, J. A., et al. (2012) · Autophagy · doi:10.4161/auto.19496 · Cited by 3,996
Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citation
Metadata
- Authors
- Geng, J., & Klionsky, D. J.
- Venue
- Autophagy
- Publisher
- Informa UK Limited
- Published
- 2010-01
- Volume
- 6
- Issue
- 1
- Pages
- 144-147
- ISSN
- 1554-8627
- 1554-8635
- PMC
- PMC2841983
- Cited by
- 12
- References
- 0
- Language
- en
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