Induction of genuine autophagy by cationic lipids in mammalian cells
Open accessAbstract
Autophagy is a cellular stress response that results in the activation of a lysosomal degradation pathway. In this report, we showed that cationic lipids, a common-used class of transfection reagents, induced genuine autophagy in mammalian cells. Extensive LC3 dot formation was observed by treatment with cationic lipids (with or without DNA), but not neutral lipids, in a HeLa cell line stably expressing GFP-LC3 (HeLa-LC3). Further proofs for autophagy were obtained by the co-localization of the LC3 dots with lysosome-specific staining patterns, observation of LC3-I to LC3-II form conversion and appearance of autophagic vacuoles under TEM. The autophagic flux assay with bafilomycin A1 and degradation of p62/SQSTM1 suggested that the autophagy induced by cationic lipids was primarily due to increased formation of autophagosomes and not decreased turnover. Moreover, cationic lipids induced autophagy in an mTOR-independent manner.
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The cationic lipids used for siRNA-mediated depletion are known to induce autophagy.
“The cationic lipids used for the siRNA-mediated depletion are known to induce autophagy34”
From A BET family protein degrader provokes senolysis by targeting NHEJ and autophagy in senescent cells (2020) · cites this paper as [34]
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References 30 · Cited by 70
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- Authors
- Man, N., Chen, Y., Zheng, F., Zhou, W., & Wen, L.-P.
- Affiliations
- University of Science and Technology of China (CN)
- Venue
- Autophagy
- Publisher
- Informa UK Limited
- Published
- 2010-05-16
- Volume
- 6
- Issue
- 4
- Pages
- 449-454
- ISSN
- 1554-8627 (ISSN-L)
- 1554-8635
- OpenAlex
- W2041092972
- Cited by
- 70
- References
- 30
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 5 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 5 · 2019: 4 · 2018: 8 · 2017: 5 · 2016: 6 · 2015: 6 · 2014: 6 · 2013: 2 · 2012: 1
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Update notices
- correction (2016-02-22)
- Open access
- bronze
- doi.org
- Locations
- Autophagy (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30721002, 30830036, 2009CB930300)
- Chinese Academy of Sciences (KSCX2-YW-R-139, KSCX2-YW-R)
- National Key Research and Development Program of China (2009CB930300)
- Topics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- autophagy induction, cationic lipids, transfection reagents, autophagosome formation, autophagic flux, mTOR-independent autophagy, lysosomal degradation, GFP-LC3
- MeSH terms
- Animals, Autophagy, Cations, HeLa Cells, Humans, Lipids, Mammals, Microtubule-Associated Proteins, Phagosomes, Green Fluorescent Proteins, Mice
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