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A reporter cell system to monitor autophagy based on p62/SQSTM1
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“GFP-SQSTM1 displays the largest magnitude change following the induction of autophagy by amino acid deprivation or rapamycin treatment, and may thus be a better marker for following autophagic flux by this method (confirmed in SH-SY5Y neuronal cell lines stably expressing GFP-SQSTM1; E.M.”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [497]
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“Similarly, a stable cell line expressing EGFP-tagged SQSTM1 under the control of an inducible promoter can be used to assess the rates of SQSTM1 degradation, taking into account the limitations outlined above (see Autophagic flux determination using flow and multispectral imaging cytometry) [497].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [497]
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Research works printed but not linked: 33
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Metadata
- Authors
- Larsen, K. B., Lamark, T., Øvervatn, A., Harneshaug, I., Johansen, T., & Bjørkøy, G.
- Venue
- Autophagy
- Publisher
- Informa UK Limited
- Published
- 2010-08-16
- Volume
- 6
- Issue
- 6
- Pages
- 784-793
- ISSN
- 1554-8627
- 1554-8635
- Cited by
- 143
- References
- 0
- Language
- en
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