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Does bafilomycin A 1 block the fusion of autophagosomes with lysosomes?
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Crosstalk between the proteasomal and autophagic systems does not appreciably affect the results obtained in the long-lived protein degradation assay.
“thus suggesting that the crosstalk between the proteasomal and autophagic systems does not appreciably affect the results obtained in the long-lived protein degradation assay (although this does not exclude the possibility that this may occur under other conditions, so this needs to be tested from case to case).”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [218]
3-MA could have some autophagy-independent effects in particular settings.
“In addition, because 3-MA could have some autophagy-independent effects in particular settings it is advisable to verify that the 3-MA-sensitive degradation is also sensitive to specific class III PtdIns3K inhibitors such as SAR-405, and to general lysosomal inhibitors (such as NH4Cl or leupeptin) [26,218].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [218]
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Fass, E., Shvets, E., Degani, I., Hirschberg, K., & Elazar, Z. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m607031200 · Cited by 286
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Research works printed but not linked: 6
- Jahreiss L, Menzies FM, Rubinsztein DC. The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes. Traffic 2008; 9:574-87.
- Kawai A, Uchiyama H, Takano S, Nakamura N, Ohkuma S. Autophagosome-lysosome fusion depends on the pH in acidic compartments in CHO cells. Autophagy 2007; 3:154-7.
- Mousavi SA, Kjeken R, Berg TO, Seglen PO, Berg T, Brech A. Effects of inhibitors of the vacuolar proton pump on hepatic heterophagy and autophagy. Biochim Biophy Acta 2001; 1510:243-57.
- Clague MJ, Urbe S, Aniento F, Gruenberg J. Vacuolar ATPase activity is required for endo- somal carrier vesicle formation. J Biol Chem 1994; 269:21-4.
- van Deurs B, Holm PK, Sandvig K. Inhibition of the vacuolar H+-ATPase with bafilomycin reduces delivery of internalized molecules from mature multivesicular endosomes to lyso- somes in HEp-2 cells. Eur J Cell Biol 1996; 69:343-50.
- van Weert AWM, Dunn KW, Geuze HJ, Maxfield FR, Stoorvogel W. Transport from late endosomes to lysosomes, but not sorting of integral membrane proteins in endosomes, depends on the vacuolar proton pump. J Cell Biol 1995; 130:821-34.
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Metadata
- Authors
- Klionsky, D. J., Elazar, Z., Seglen, P. O., & Rubinsztein, D. C.
- Venue
- Autophagy
- Publisher
- Informa UK Limited
- Published
- 2008-08-22
- Volume
- 4
- Issue
- 7
- Pages
- 849-850
- ISSN
- 1554-8627
- 1554-8635
- Cited by
- 447
- References
- 2
- Language
- en
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