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Improved Flow Cytometric Analysis of the Budding Yeast Cell Cycle
Open accessAbstract
The budding yeast, Saccharomyces cerevisiae has been a remarkably useful model system for the study of eukaryotic cell cycle regulation. Flow cytometric analysis of DNA content in budding yeast has become a standard tool for the analysis of cell cycle progression. However, popular protocols utilizing the DNA binding dye, propidium iodide, suffer from a number of drawbacks that confound accurate analysis by flow cytometry. Here we show the utility of the DNA binding dye, SYTOX Green, in the cell cycle analysis of yeast. Samples analyzed using SYTOX Green exhibited better coefficients of variation, improved linearity between DNA content and fluorescence, and decreased peak drift associated with changes in dye concentration, growth conditions or cell size.
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Cited by
Prions are a common mechanism for phenotypic inheritance in wild yeasts
Halfmann, R., Jarosz, D. F., Jones, S. K., Chang, A., Lancaster, A. K., & Lindquist, S. (2012) · Nature · doi:10.1038/nature10875 · Cited by 401
Sheu, Y.-J., & Stillman, B. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.07.033 · Cited by 340
Chang, Y. C., Khanal Lamichhane, A., & Kwon-Chung, K. J. (2018) · mBio · doi:10.1128/mbio.01290-18 · Cited by 34
Bélanger, F., Angers, J.-P., Fortier, É., Hammond-Martel, I., Costantino, S., Drobetsky, E., & Wurtele, H. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.685883 · Cited by 24
Metadata
- Authors
- Haase, S. B., & Reed, S. I.
- Venue
- Cell Cycle
- Publisher
- Informa UK Limited
- Published
- 2002-03
- Volume
- 1
- Issue
- 2
- Pages
- 117-121
- ISSN
- 1538-4101 (ISSN-L)
- 1551-4005
- OpenAlex
- W2603569803
- Cited by
- 240
- References
- 0
- FWCI
- 1.30
- Type
- journal article
- Language
- en
- Open access
- bronze
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