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Cell Cycle Synchronization at the G2/M Phase Border by Reversible Inhibition of CDK1
Open accessAbstract
Chemical agents for cell cycle synchronization have greatly facilitated the study of biochemical events driving cell cycle progression. G1, S and M phase inhibitors have been developed and used widely in cell cycle research. However, currently there are no effective G2 phase inhibitors and synchronization of cultured cells in G2 phase has been challenging. Recently, a selective CDK1 inhibitor, RO-3306, has been identified that reversibly arrests proliferating human cells at the G2/M phase border and provides a novel means for cell cycle synchronization. A single-step protocol using RO-3306 permits the synchronization of >95% of cycling cancer cells in G2 phase. RO-3306 arrested cells enter mitosis rapidly after release from the G2 block thus allowing for isolation of mitotic cells without microtubule poisons. RO-3306 represents a new molecular tool for studying CDK1 function in human cells.
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Cited by
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McCloy, R. A., Rogers, S., Caldon, C. E., Lorca, T., Castro, A., & Burgess, A. (2014) · Cell Cycle · doi:10.4161/cc.28401 · Cited by 853
Chromosome Segregation Errors as a Cause of DNA Damage and Structural Chromosome Aberrations
Janssen, A., van der Burg, M., Szuhai, K., Kops, G. J. P. L., & Medema, R. H. (2011) · Science · doi:10.1126/science.1210214 · Cited by 579
Cell-Cycle Regulation Accounts for Variability in Ki-67 Expression Levels
Sobecki, M., Mrouj, K., Colinge, J., Gerbe, F., Jay, P., Krasinska, L., Dulic, V., & Fisher, D. (2017) · Cancer Research · doi:10.1158/0008-5472.can-16-0707 · Cited by 375
Banerjee, S., Zagórska, A., Deak, M., Campbell, D. G., Prescott, A. R., & Alessi, D. R. (2014) · Biochemical Journal · doi:10.1042/bj20140408 · Cited by 32
The mTORC1/S6K/PDCD4/eIF4A Axis Determines Outcome of Mitotic Arrest
Moustafa-Kamal, M., Kucharski, T. J., El-Assaad, W., Abbas, Y. M., Gandin, V., Nagar, B., Pelletier, J., Topisirovic, I., & Teodoro, J. G. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108230 · Cited by 31
Metadata
- Authors
- Vassilev, L. T.
- Venue
- Cell Cycle
- Publisher
- Informa UK Limited
- Published
- 2006-11-08
- Volume
- 5
- Issue
- 22
- Pages
- 2555-2556
- ISSN
- 1538-4101 (ISSN-L)
- 1551-4005
- OpenAlex
- W2151602082
- Cited by
- 198
- References
- 0
- FWCI
- 0.87
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Vassilev, L. T. (2006). Cell Cycle Synchronization at the G2/M Phase Border by Reversible Inhibition of CDK1. Cell Cycle. https://doi.org/10.4161/cc.5.22.3463
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