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Cited by 7Pancreatic function and diabetesCellular transport and secretionIon channel regulation and function

Phasic and tonic modes of depolarization-exocytosis coupling in β-cells of porcine islets of Langerhans

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Misler, Stanley, Silva, Amelia M., Barnett, David W., Dickey, Adam S.·2009·Channels
DOI 10.4161/chan.3.2.7866
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Abstract

In response to depolarizations that open voltage dependent Ca2+ channels single porcine beta-cells display heterogeneous time courses of exocytosis. Some cells display phasic exocytosis that is triggered by individual or short burst of action potentials typically characteristic of glucose-induced electrical activity or brief voltage clamp depolarization. Other cells, singularly or additionally, display tonic exocytosis that (i) is triggered during prolonged (up to seconds-long) depolarizations to voltages (-30 to -20 mV), and (ii) coincides with rises in global cytosolic [Ca2+] >500 nM. We suggest that tonic exocytosis (i) likely results from a recently described pool of granules that is more Ca2+ sensitive and less co-localized with voltage-sensitive Ca2+ entry channels than that contributing to phasic exocytosis and (ii) helps tune exocytosis to glucose-induced electrical activity when the latter consists of spike activity followed by intervals of plateau depolarization to nearly -20 mV.

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  • Canine and porcine β-cells show no obvious oscillatory electrical activity in membrane potential recordings.

    “Membrane potential recordings have also been performed on canine and porcine β-cells (454, 455). These revealed no obvious oscillatory electrical activity but as these measurements were made in dispersed β-cells, it remains unclear whether this reflects a true species difference or if it is simply a consequence of cell isolation (as in the mouse).”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [454]

    The Results (pp. 5–6) report membrane-potential recordings from porcine β-cells showing irregular action potentials and intermittent plateau depolarizations, rather than describing a regular oscillatory pattern. The Discussion (p. 8) confirms that recordings were made from isolated cells and cautions against extrapolating to intact islets. The paper does not explicitly conclude that porcine cells show no obvious oscillatory electrical activity; the canine portion is not the part this citation appears to support. (LLM, conf=0.76)

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Research works printed but not linked: 31

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Cited by

Sorted by citations

Authors
Misler, S., Silva, A. M., Barnett, D. W., & Dickey, A. S.
Affiliations
  • Washington University Medical Center (US)
  • Washington University in St. Louis (US)
Corresponding author
Misler, Stanley
ORCID
Venue
Channels
Publisher
Informa UK Limited
Published
2009-03-15
Volume
3
Issue
2
Pages
101-109
ISSN
  • 1933-6950 (ISSN-L)
  • 1933-6969
DOI
10.4161/chan.3.2.7866
OpenAlex
W2022344671
Cited by
7
References
2
Citations by year
2017: 1 · 2013: 1 · 2012: 1
FWCI
1.60
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Channels (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Digital Commons@Becker (Washington University School of Medicine) (submittedVersion)
Funders
  • Washington University in St. Louis
  • Fundação Luso-Americana para o Desenvolvimento
  • National Institutes of Health (DK20579)
  • National Institute of General Medical Sciences (T32GM007281)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R0I-DK20579, R01DK037380)
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
pancreatic beta cells, pancreatic islets, exocytosis, depolarization, voltage-gated calcium channels, intracellular calcium, action potential, pigs
MeSH terms
Action Potentials, Animals, Calcium, Electrophysiology, Exocytosis, Glucose, Islets of Langerhans, Membrane Potentials, Swine, Calcium Channels, Insulin-Secreting Cells

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