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Analytical methods for physicochemical characterization of antibody drug conjugates
Open accessAbstract
Antibody-drug conjugates (ADCs), formed through the chemical linkage of a potent small molecule cytotoxin (drug) to a monoclonal antibody, have more complex and heterogeneous structures than the corresponding antibodies. This review describes the analytical methods that have been used in their physicochemical characterization. The selection of the most appropriate methods for a specific ADC is heavily dependent on the properties of the linker, the drug, and the choice of attachment sites (lysines, inter-chain cysteines, Fc glycans). Improvements in analytical techniques such as protein mass spectrometry and capillary electrophoresis have significantly increased the quality of information that can be obtained for use in product and process characterization, and for routine lot release and stability testing.
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Cited by
Antibody–Drug Conjugates: An Emerging Concept in Cancer Therapy
Chari, R. V. J., Miller, M. L., & Widdison, W. C. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201307628 · Cited by 930
Antibody–Drug Conjugates (ADCs) for Personalized Treatment of Solid Tumors: A Review
Lambert, J. M., & Morris, C. Q. (2017) · Advances in Therapy · doi:10.1007/s12325-017-0519-6 · Cited by 304
Fekete, S., Guillarme, D., Sandra, P., & Sandra, K. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.5b04561 · Cited by 258
Metadata
- Authors
- Wakankar, A., Chen, Y., Gokarn, Y., & Jacobson, F. S.
- Venue
- mAbs
- Publisher
- Informa UK Limited
- Published
- 2011-03
- Volume
- 3
- Issue
- 2
- Pages
- 161-172
- ISSN
- 1942-0862 (ISSN-L)
- 1942-0870
- OpenAlex
- W2073176225
- Cited by
- 382
- References
- 0
- FWCI
- 6.23
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wakankar, A., Chen, Y., Gokarn, Y., & Jacobson, F. S. (2011). Analytical methods for physicochemical characterization of antibody drug conjugates. mAbs. https://doi.org/10.4161/mabs.3.2.14960
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