Molecular and Biological Diagnostic Tests for Monitoring Benzimidazole Resistance in Human Soil-Transmitted Helminths
Open accessAbstract
In endemic countries with soil-transmitted helminths mass drug administration with albendazole or mebendazole are being implemented as a control strategy. However, it is well known in veterinary helminths that the use of the same benzimidazole drugs can place selection on the β-tubulin gene, leading to resistance. Given the concern that resistance could arise in human soil-transmitted helminths, there is an urgent need to develop accurate diagnostic tools for monitoring resistance. In this study, we developed molecular assays to detect putative resistance genetic changes in Ascaris lumbricoides, Trichuris trichiura, and hookworms, and we optimized an egg hatch assay for the canine hookworm Ancylostoma caninum and applied it to Necator americanus. Both assays were tested on field samples. The molecular assays demonstrated their reproducibility and capacity to detect the presence of worms carrying putative resistance-associated genetic changes. However, further investigations are needed to validate our molecular and biological tests on additional field isolates.
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The EHA was assessed in A. caninum and used to evaluate drug susceptibility/resistance to benzimidazoles in the human hookworm Necator americanus.
“Additionally, the EHA was assessed in A. caninum [40] and used to evaluate drug susceptibility/resistance to benzimidazoles in the human hookworm, Necator americanus [40, 58, 59].”
From Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat? (2019) · cites this paper as [40]
The IC50 values measured for the two susceptible isolates tested were very similar to that previously reported for A. caninum.
“The IC50 values we measured for the two susceptible isolates we tested were very similar to that previously reported for A. caninum [40]”
From Multiple drug resistance in the canine hookworm Ancylostoma caninum: an emerging threat? (2019) · cites this paper as [40]
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- Authors
- Diawara, A., Schwenkenbecher, J. M., Kaplan, R. M., & Prichard, R. K.
- Affiliations
- McGill University (CA)
- Venue
- The American Society of Tropical Medicine and Hygiene
- Published
- 2013-06-05
- Volume
- 88
- Issue
- 6
- Pages
- 1052-1061
- ISSN
- 0002-9637 (ISSN-L)
- 1476-1645
- PMC
- PMC3752802
- OpenAlex
- W2111191097
- Cited by
- 78
- References
- 39
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 7 · 2023: 3 · 2022: 9 · 2021: 6 · 2020: 4 · 2019: 8 · 2018: 10 · 2017: 7 · 2016: 5 · 2015: 6 · 2014: 7 · 2013: 3
- FWCI
- 5.92
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- American Journal of Tropical Medicine and Hygiene (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Keywords
- benzimidazole resistance, soil-transmitted helminths, albendazole, mebendazole, beta-tubulin gene, Ascaris lumbricoides, Trichuris trichiura, hookworm, Ancylostoma caninum, Necator americanus, egg hatch assay, molecular detection of resistance, mass drug administration, anthelmintic resistance monitoring
- MeSH terms
- Ancylostoma, Ancylostomatoidea, Animals, Anthelmintics, Benzimidazoles, Diagnostic Tests, Routine, Dogs, Drug Resistance, Gene Frequency, Helminthiasis, Helminths, Host-Parasite Interactions, Humans, Mutation, Trichuris, Tubulin, Reproducibility of Results, Albendazole, Ascaris lumbricoides, Necator americanus, Sequence Analysis, DNA, DNA, Helminth, Polymorphism, Single Nucleotide
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