A Novel, Multi-Parallel, Real-Time Polymerase Chain Reaction Approach for Eight Gastrointestinal Parasites Provides Improved Diagnostic Capabilities to Resource-Limited At-Risk Populations

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Direct detection of Strongyloides infection via molecular and antigen detection methods

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Parasitological versus molecular diagnosis of strongyloidiasis in serial stool samples: how many?

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Parasitic infections represent a significant health threat among recent immigrants in Chicago

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Capillaria Ova and Diagnosis of Trichuris trichiura Infection in Humans by Kato-Katz Smear, Liberia

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Detection of Giardia lamblia by Microscopic Examination, Rapid Chromatographic Immunoassay Test, and Molecular Technique

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Human Trichuriasis: Diagnostics Update

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The laboratory diagnosis of Strongyloides stercoralis

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Detection of six soil-transmitted helminths in human stool by qPCR- a systematic workflow

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Towards soil-transmitted helminths transmission interruption: The impact of diagnostic tools on infection prediction in a low intensity setting in Southern Mozambique

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Parasitic Contamination of Soil in the Southern United States

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A hospital qPCR-based survey of 10 gastrointestinal parasites in routine diagnostic screening, Marseille, France

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Strongyloides stercoralis: current perspectives

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Soil-Transmitted Helminths in Children in a Remote Aboriginal Community in the Northern Territory: Hookworm is Rare but Strongyloides stercoralis and Trichuris trichiura Persist

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Simplified protocol for DNA extraction and amplification of 2 molecular markers to detect and type Giardia duodenalis

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Use of Multi-Parallel Real-Time Quantitative PCR to Determine Blastocystis Prevalence and Association with Other Gastrointestinal Parasite Infection in a Rural Honduran Location

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Molecular Tools for Diagnosis and Surveillance of Soil-Transmitted Helminths in Endemic Areas

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Effective Laboratory Diagnosis of Parasitic Infections of the Gastrointestinal Tract: Where, When, How, and What Should We Look For?

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Molecular Biology Can Change the Classic Laboratory Approach for Intestinal Protozoan Infections

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A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)

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Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium spp. in Stool Samples

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Evaluation of School Children Nutritional Status in Ecuador Using Nutrimetry: A Proposal of an Education Protocol to Address the Determinants of Malnutrition

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Drug Resistance Assays for Parasitic Diseases

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Soil surveillance for monitoring soil-transmitted helminths: Method development and field testing in three countries

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Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites Giardia intestinalis, Entamoeba spp., and Cryptosporidium spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays

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Exploring biocultural determinants of intestinal health: Do resource access and parasite exposure contribute to intestinal inflammation among a preliminary sample of children in rural Mississippi?

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Molecular diagnosis of infectious diarrhea: focus on enteric protozoa

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Strongyloides stercoralis and Relatives: Recent Advances in General and Molecular Biology

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Optimizing DNA Extraction from Pediatric Stool for Diagnosis of Tuberculosis and Use in Next-Generation Sequencing Applications

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Calculating the prevalence of soil-transmitted helminth infection through pooling of stool samples: Choosing and optimizing the pooling strategy

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A stool based qPCR for the diagnosis of TB in children and people living with HIV in Uganda, Eswatini and Mozambique (Stool4TB): a protocol for a multicenter diagnostic evaluation

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Development of a Droplet Digital PCR for Detection of Trichuriasis in Sheep

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Molecular Detection of Soil-Transmitted Helminths and Enteric Protozoa Infection in Children and Its Association with Household Water and Sanitation in Manhiça District, Southern Mozambique

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Case Report: Molecular Diagnosis of Cystoisospora belli in a Severely Immunocompromised Patient with HIV and Kaposi Sarcoma

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Molecular detection and characterization of Giardia spp., Cryptosporidium spp., and Blastocystis in captive wild animals rescued from central Colombia

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Effects of ivermectin mass drug administration for malaria vector control on ectoparasites and soil-transmitted helminths: a cluster randomized trial

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Epidemiology of giardiasis and assemblages A and B and effects on diarrhea and growth trajectories during the first 8 years of life: Analysis of a birth cohort in a rural district in tropical Ecuador

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Geographic Distribution of Human Infections with Zoonotic Ancylostoma ceylanicum and Anthropophilic Hookworms in Ecuador: A Retrospective Analysis of Archived Stool Samples

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Rectal Swabs as an Alternative Sample Collection Method to Bulk Stool for the Real-Time PCR Detection of Giardia duodenalis

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Diagnostic Potential of an IgE-ELISA in Detecting Strongyloidiasis

Ahmad, H., Balachandra, D., Arifin, N., Nolan, T. J., Lok, J. B., Khan, A. H., Yunus, M. H., & Noordin, R. (2020) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.20-0265 · Cited by 8

Sensitive and semiquantitative detection of soil-transmitted helminth infection in stool using a recombinase polymerase amplification-based assay

Cantera, J. L., White, H. N., Forrest, M. S., Stringer, O. W., Belizario, V. Y., Storey, H. L. G., de Hostos, E. L., & Santos, T. D. L. (2021) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0009782 · Cited by 8

Helminth Infections in Children

Sanchez‐Vegas, C., & Villavicencio, K. L. H. (2022) · Pediatrics in Review · doi:10.1542/pir.2020-001347 · Cited by 8

Prevalence of intestinal parasites and comparison of detection techniques for soil-transmitted helminths among newly arrived expatriate labors in Jeddah, Saudi Arabia

Al-Refai, M. F., & Wakid, M. H. (2024) · PeerJ · doi:10.7717/peerj.16820 · Cited by 9

Comparative evaluation of real-time PCR and ELISA for the detection of human fascioliasis

Bakhshipour, F., Zibaei, M., Rokni, M. B., Miahipour, A., Firoozeh, F., Beheshti, M., Beikzadeh, L., Alizadeh, G., Aryaeipour, M., & Raissi, V. (2024) · Scientific Reports · doi:10.1038/s41598-024-54602-y · Cited by 7

Comparison of multi-parallel quantitative real-time PCRs targeting different DNA regions and detecting soil-transmitted helminths in stool

Papaiakovou, M., Cimino, R. O., Pilotte, N., Dunn, J. C., Littlewood, D. T. J., Williams, S. A., Krolewiecki, A. J., & Mejía, R. (2024) · Parasites & Vectors · doi:10.1186/s13071-024-06464-6 · Cited by 7

Evaluation of loop mediated isothermal amplification, quantitative real-time PCR, conventional PCR methods for identifying Ascaris lumbricoides in human stool samples

Thakur, M., Mewara, A., Lakshmi, P. V. M., Guleria, S., & Khurana, S. (2025) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2025.116808 · Cited by 7

Egg excretion patterns of soil-transmitted helminth infections in humans following albendazole-ivermectin and albendazole treatment

Welsche, S., Schneeberger, P. H. H., Hattendorf, J., Sayasone, S., Hürlimann, E., & Keiser, J. (2024) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0012073 · Cited by 6

Clustering of Gastrointestinal Microorganisms in Human Stool Samples from Ghana

Backhaus, J., Kann, S., Hahn, A., Weinreich, F., Blohm, M., Tanida, K., Feldt, T., Sarfo, F. S., Cristanziano, V. D., Loderstädt, U., et al. (2024) · Pathogens · doi:10.3390/pathogens13070583 · Cited by 7

Rationale for Quality Assurance in Fecal Egg Monitoring of Soil-Transmitted Helminthiasis

Hoekendijk, D. J. L., Hill, P. C., & Sowerby, S. J. (2016) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.15-0463 · Cited by 7

Utilization of real time PCR for the assessment of egg burden in the organs of Schistosoma japonicum experimentally infected mice

Dang-Trinh, M.-A., Angeles, J. M. M., Moendeg, K. J., Macalanda, A. M. C., Higuchi, L., Oto, C., Kirinoki, M., Chigusa, Y., & Kawazu, S. (2018) · Experimental Parasitology · doi:10.1016/j.exppara.2018.04.014 · Cited by 7

Precise Dispensing Technology Using Electroformed Tubes for Micro-Volume Blood Diagnosis

Ando, T., Hirano, M., Ishige, Y., & Adachi, S. (2018) · IEEE Journal of Translational Engineering in Health and Medicine · doi:10.1109/jtehm.2018.2852664 · Cited by 7

Molecular Diagnostics in the Diagnosis of Parasitic Infection

Pritt, B. S. (2015) · Methods in microbiology · doi:10.1016/bs.mim.2015.05.001 · Cited by 7

Molecular and non-molecular approaches to etiologic diagnosis of gastroenteritis

Zhang, X., Das, S., Dunbar, S. A., & Tang, Y. (2020) · Advances in clinical chemistry · doi:10.1016/bs.acc.2020.02.007 · Cited by 7

Whip-LAMP: a novel LAMP assay for the detection of Trichuris muris-derived DNA in stool and urine samples in a murine experimental infection model

Fernández‐Soto, P., Fernández-Medina, C., Cruz-Fernández, S., Crego-Vicente, B., Febrer‐Sendra, B., Diego, J. G.-B., Gorgojo-Galindo, Ó., López‐Abán, J., Vicente-Santiago, B., & Muro, A. (2020) · Parasites & Vectors · doi:10.1186/s13071-020-04435-1 · Cited by 7

Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs

Gandasegui, J., Grau-Pujol, B., Cambra-Pellejà, M., Escola, V., Demontis, M. A., Cossa, A., Jamine, J. C., Balaña‐Fouce, R., van Lieshout, L., Muñóz, J., et al. (2021) · Parasites & Vectors · doi:10.1186/s13071-021-04941-w · Cited by 7

Introduction to Helminth Infections

Maguire, J. H. (2015) · Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases · doi:10.1016/b978-1-4557-4801-3.00287-3 · Cited by 7

Performance of real-time polymerase chain reaction and Kato-Katz for diagnosing soil-transmitted helminth infections and evaluating treatment efficacy of emodepside in randomized controlled trials

Lotz, C. N., Mrimi, E. C., Schneeberger, P. H. H., Ali, S. M., Hattendorf, J., & Keiser, J. (2025) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0012872 · Cited by 6

Epidemiology of intestinal parasite infections and multiparasitism and their impact on growth and hemoglobin levels during childhood in tropical Ecuador: A longitudinal study using molecular detection methods

Mejía, R., Ster, I. C., Chico, M. E., Guadalupe, I., Arévalo‐Cortes, A., Lopez, A., Oviedo, A. Y., & Cooper, P. J. (2025) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0013004 · Cited by 6

A comparison of the diagnostic capability of Kato-Katz and real-time PCR for the assessment of treatment efficacy of ivermectin and albendazole combination against T. trichiura infections

Matamoros, G., Sanchez, A. L., Cimino, R. O., Krolewiecki, A. J., & Mejía, R. (2024) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0012677 · Cited by 6

Development and validation of a high-throughput qPCR platform for the detection of soil-transmitted helminth infections

Pilotte, N., Omballa, V., Voss, M., Padgett, L. R., Manuel, M., Goodman, J., Littlewood, D. T. J., Manoharan, Z. Z., van Lieshout, L., Verweij, J. J., et al. (2025) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0012760 · Cited by 6

Soil surveillance for monitoring soil-transmitted helminth infections: method development and field testing in three countriesPreprint

Manuel, M., Amato, H. K., Pilotte, N., Chieng, B., Araka, S. B., Siko, E. J., Harris, M., Nadimpalli, M. L., Janagaraj, V., Houngbégnon, P., et al. (2023) · medRxiv · doi:10.1101/2023.09.26.23296174 · Cited by 6

Assessment of the role of gut health in childhood stunting in a multisite, longitudinal study in India, Indonesia and Senegal: a UKRI GCRF Action Against Stunting Hub protocol

Kadia, B. M., Ramsteijn, A. S., Dasi, T., Fahmida, U., Kulkarni, B., Faye, B. T., Htet, M. K., Sow, D., Kalashikam, R. R., Sharma, R., et al. (2024) · BMJ Paediatrics Open · doi:10.1136/bmjpo-2022-001637 · Cited by 6

Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico

Aguayo-Patrón, S., Castillo-Fimbres, R. S., Quihui‐Cota, L., & de la Barca, A. M. C. (2017) · The Journal of Infection in Developing Countries · doi:10.3855/jidc.9350 · Cited by 5

Frequency and distribution of Blastocystis sp. subtypes in patients with spondyloarthritis in Bogotá, Colombia

Hernández, P. C., Morales, L., Chaparro‐Olaya, J., Ávila, J. D., Bautista‐Molano, W., Bello-Gualtero, J. M., Beltrán-Ostos, A., & Romero‐Sánchez, C. (2021) · Parasite Epidemiology and Control · doi:10.1016/j.parepi.2021.e00227 · Cited by 5

Near-infrared spectroscopy and machine learning algorithms for rapid and non-invasive detection of Trichuris

Kariyawasam, T. N., Ciocchetta, S., Visendi, P., Magalhães, R. J. S., Smith, M. E., Giacomin, P., & Sikulu-Lord, M. T. (2023) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0011695 · Cited by 5

Parasite contamination of soil in different Peruvian locations and outside built environments

Pineda, C., Rivera, M. D. C., Montalvo-Sabino, E., Mejia, L. E., Keegan, K. E., Alvarez, L. P. P., Mora, J. J., Vega, F. E., Mejia, E. R., Herrera, P. O., et al. (2025) · Parasites & Vectors · doi:10.1186/s13071-025-06762-7 · Cited by 4

Molecular Testing of Environmental Samples as a Potential Source to Estimate Parasite Infection

Mejía, R., Slatko, B. E., Almazán, M. C., Cimino, R. O., Krolewiecki, A. J., Duran, N. M., Aspetty, J. E. V., Vargas, P. A., Amorim, C. C. O., Geiger, S. M., et al. (2024) · Tropical Medicine and Infectious Disease · doi:10.3390/tropicalmed9100226 · Cited by 4

Efficacy and safety of moxidectin and albendazole compared to ivermectin and albendazole co-administration in adolescents infected with Trichuris trichiura: a randomized controlled trial protocol

Welsche, S., Mrimi, E. C., Keller, L., Hürlimann, E., Hofmann, D., Hattendorf, J., Ali, S. M., & Keiser, J. (2021) · Gates Open Research · doi:10.12688/gatesopenres.13299.2 · Cited by 4

Differential gut microbiome in spondyloarthritis patients associated to Blastocystis colonization

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Occurrence of parasites in waters used for crops irrigation and vegetables from the Savannah of Bogotá, Colombia

Santos, D. E. O., Zapata, Y. A. U., Buitrago, C. A., Herrera, G. S., Becoche, L. E. C., López, M. C., Bustos-López, M. C., & Pineda, C. O. (2024) · Environmental Science and Pollution Research · doi:10.1007/s11356-024-33088-1 · Cited by 4

Strongyloidiasis as a Cause of Chronic Diarrhea, Identified Using Next-Generation Strongyloides stercoralis-Specific Immunoassays

Thamwiwat, A., Mejía, R., Nutman, T. B., & Bates, J. T. (2014) · Current Tropical Medicine Reports · doi:10.1007/s40475-014-0026-7 · Cited by 4

Poverty associated with the environmental contamination of gastrointestinal parasites in the Southern United StatesPreprint

Blackburn, C. C., Yan, S. M., McCormick, D. W., Herrera, L. N., Iordanov, R. B., Bailey, M. D., Bottazzi, M. E., Hotez, P. J., & Mejía, R. (2023) · medRxiv · doi:10.1101/2023.01.10.23284404 · Cited by 4

1789Multi-parallel quantitative real-time PCR surveillance of gastrointestinal parasites in a symptomatic rural Argentinian population: initial results of the Latin American Multicenter Parasite Study (LAMPS)

Mejía, R., Vargas, P. A., Jeun, R., Krolewiecki, A. J., Juaréz, M., Cajal, P. S., Nasser, J. R., Hotez, P. J., & Cimino, R. O. (2014) · Open Forum Infectious Diseases · doi:10.1093/ofid/ofu051.161

Identification of human intestinal parasites affecting an asymptomatic peri-urban Argentinian population using multi-parallel quantitative real-time polymerase chain reaction

Cimino, R. O., Jeun, R., Juaréz, M., Cajal, P. S., Vargas, P. A., Echazú, A., Bryan, P. E., Nasser, J. R., Krolewiecki, A. J., & Mejía, R. (2015) · Parasites & Vectors · doi:10.1186/s13071-015-0994-z · Cited by 92

Performance of Real-Time Polymerase Chain Reaction Assays for the Detection of 20 Gastrointestinal Parasites in Clinical Samples from Senegal

Sow, D., Parola, P., Sylla, K., Ndiaye, M., Delaunay, P., Halfon, P., Camiade, S., Dieng, T., Tine, R. C. K., Faye, B. T., et al. (2017) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.16-0781 · Cited by 41

Multiplex Real-Time PCR Assay Targeting Eight Parasites Customized to the Korean Population: Potential Use for Detection in Diarrheal Stool Samples from Gastroenteritis Patients

Won, E. J., Kim, S. H., Kee, S., Shin, J. H., Suh, S., Chai, J.-Y., Ryang, D. W., & Shin, M. (2016) · PLoS ONE · doi:10.1371/journal.pone.0166957 · Cited by 37

Multiplex PCR for gastrointestinal parasites in stool: Benchmarking against direct microscopy and simplex PCR

Garg, P., Bhasin, S. L., Malhotra, P., Rana, S. S., Singh, S., Sethi, J., Sehgal, R., Khurana, S., & Datta, P. (2024) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2024.116475 · Cited by 10