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A Method for the Isolation and Identification of Dengue Viruses, using Mosquito Cell Cultures
Abstract
An improved method for the isolation and identification of dengue viruses is described. Viruses were isolated in mosquito cell cultures (C6/36 or AP-61), identified by indirect fluorescent antibody technique, and typed by complement-fixation test, using the cell culture fluid as antigen. The sensitivity of this method was compared with mosquito inoculation in comparative titrations of 16 low passage dengue virus strains. Although lower virus titers were obtained by the mosquito cell culture technique, its decreased sensitivity was compensated for by the much larger volume (588X) which could be assayed. By incubating the mosquito cells at 32 degrees C, dengue viruses can be identified and typed within 6 days after inoculation.
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Cited by
Dengue and dengue haemorrhagic fever
Rigau-Pérez, J. G., Clark, G. G., Gubler, D. J., Reiter, P., Sanders, E. J., & Vance Vorndam, A. (1998) · The Lancet · doi:10.1016/s0140-6736(97)12483-7 · Cited by 1,051
Molecular evolution and distribution of dengue viruses type 1 and 2 in nature
Rico-Hesse, R. (1990) · Virology · doi:10.1016/0042-6822(90)90102-w · Cited by 593
Serious adverse events associated with yellow fever 17DD vaccine in Brazil: a report of two cases
Vasconcelos, P. F., Luna, E. J., Galler, R., Silva, L. J., Coimbra, T. L., Barros, V. L., Monath, T. P., Rodigues, S. G., Laval, C., Costa, Z. G., et al. (2001) · The Lancet · doi:10.1016/s0140-6736(01)05326-0 · Cited by 287
Re-emergence of epidemic Venezuelan equine encephalomyelitis in South America
Weaver, S. C., Salas, R., Rico-Hesse, R., Ludwig, G. V., Oberste, M. S., Boshell, J., & Tesh, R. B. (1996) · The Lancet · doi:10.1016/s0140-6736(96)02275-1 · Cited by 285
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Metadata
- Authors
- Tesh, R. B.
- Venue
- The American Journal of Tropical Medicine and Hygiene
- Published
- 1979-11
- Volume
- 28
- Issue
- 6
- Pages
- 1053-1059
- ISSN
- 0002-9637 (ISSN-L)
- OpenAlex
- W2336878351
- Cited by
- 192
- References
- 0
- FWCI
- 8.33
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tesh, R. B. (1979). A Method for the Isolation and Identification of Dengue Viruses, using Mosquito Cell Cultures. The American Journal of Tropical Medicine and Hygiene. https://doi.org/10.4269/ajtmh.1979.28.1053
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
