The DOI matches its Crossref record.
Rapid Identification of Dengue Virus Isolates by using Monoclonal Antibodies in an Indirect Immunofluorescence Assay
Abstract
Type-specific monoclonal antibodies prepared against the four dengue (DEN) virus serotypes were evaluated for their ability to identify low-passage human and mosquito isolates from Jamaica and West Africa by an indirect immunofluorescence assay. Serotyped human isolates from Jamaican dengue fever patients included 12 DEN-1, two DEN-2, and five DEN-4 viruses. Viruses from West Africa included 84 DEN-2 mosquito strains as well as two DEN-1 and one DEN-2 from humans. Results obtained using the immunofluorescence assay were consistent with virus identifications obtained using the more classical but costly and time-consuming plaque-reduction neutralization test. More viral isolates and higher virus yields were obtained using the C6/36 clone of Aedes albopictus cells rather than LLC-MK2 (monkey kidney) cells. Dengue type-specific monoclonal antibodies detected prototype viral antigens 24-48 hours postinfection in C6/36 cells. This is the first time that monoclonal antibodies have been used to serotype low-passage flavivirus isolates.
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Cited by
Dengue: a continuing global threat
Guzman, M. G., Halstead, S. B., Artsob, H., Buchy, P., Farrar, J., Gubler, D. J., Hunsperger, E., Kroeger, A., Margolis, H. S., Martínez, E., et al. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2460 · Cited by 2,082
Guzmán, M. G., & Kouri, G. (2002) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(01)00171-2 · Cited by 1,163
Dengue and dengue haemorrhagic fever
Rigau-Pérez, J. G., Clark, G. G., Gubler, D. J., Reiter, P., Sanders, E. J., & Vance Vorndam, A. (1998) · The Lancet · doi:10.1016/s0140-6736(97)12483-7 · Cited by 1,051
Calisher, C. H., Karabatsos, N., Dalrymple, J. M., Shope, R. E., Porterfield, J. S., Westaway, E. G., & Brandt, W. E. (1989) · Journal of General Virology · doi:10.1099/0022-1317-70-1-37 · Cited by 893
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Metadata
- Authors
- Henchal, E. A., McCown, J. M., Seguin, M. C., Gentry, M. K., & Brandt, W. E.
- Venue
- The American Journal of Tropical Medicine and Hygiene
- Published
- 1983-01
- Volume
- 32
- Issue
- 1
- Pages
- 164-169
- ISSN
- 0002-9637 (ISSN-L)
- OpenAlex
- W2342925644
- Cited by
- 162
- References
- 0
- FWCI
- 4.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Henchal, E. A., McCown, J. M., Seguin, M. C., Gentry, M. K., & Brandt, W. E. (1983). Rapid Identification of Dengue Virus Isolates by using Monoclonal Antibodies in an Indirect Immunofluorescence Assay. The American Journal of Tropical Medicine and Hygiene. https://doi.org/10.4269/ajtmh.1983.32.164
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