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Circulating CD133+/–CD34– Have Increased c-MYC Expression in Myeloproliferative Neoplasms

Uslu Bıçak, İldeniz, Tokcan, Berkay, Yavuz, Akif Selim, Tokdemir, Selçuk Sözer·2023·Turkish Journal of Hematology
DOI 10.4274/tjh.galenos.2022.2022.0343PMC PMC9979741
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Figure 1 Fluorescence‐activated cell sorting (FACS) plot of peripheral blood mononuclear cells of a patient (P29) with Philadelphia chromosome-negative Ph– myeloproliferative neoplasm with polycythemia vera using gold nanoparticle probes. For all analyses, the main population of cells was gated excluding dead and fractured cells. After settings were completed for the background and control probes, including uptake fluorescence, analysis and sorting of the gold nanoparticle‐treated cells were performed according to their signals in channels of FL‐2 for JAK2V617F‐CY3 and FL‐4 for JAK2 wild‐type (wt)‐CY5 transcripts. The FACS of the gold nanoparticle-treated cells was performed in four quadrants: JAK2V617F–JAK2wt‐ for the negative control, JAK2V617F+JAK2wt– for JAK2V617F mutant mRNA, JAK2V617F+JAK2wt+ for both JAK2wt and JAK2V617F mRNA-positive cells, and JAK2V617F−JAK2wt+ for JAK2wt mRNA.
Figure 1 Fluorescence‐activated cell sorting (FACS) plot of peripheral blood mononuclear cells of a patient (P29) with Philadelphia chromosome-negative Ph– myeloproliferative neoplasm with polycythemia vera using gold nanoparticle probes. For all analyses, the main population of cells was gated excluding dead and fractured cells. After settings were completed for the background and control probes, including uptake fluorescence, analysis and sorting of the gold nanoparticle‐treated cells were performed according to their signals in channels of FL‐2 for JAK2V617F‐CY3 and FL‐4 for JAK2 wild‐type (wt)‐CY5 transcripts. The FACS of the gold nanoparticle-treated cells was performed in four quadrants: JAK2V617F–JAK2wt‐ for the negative control, JAK2V617F+JAK2wt– for JAK2V617F mutant mRNA, JAK2V617F+JAK2wt+ for both JAK2wt and JAK2V617F mRNA-positive cells, and JAK2V617F−JAK2wt+ for JAK2wt mRNA.CC BY-NC-ND · PubMed Central
Figure 2 Relative c-MYC gene expression analysis of (A) mononuclear cells (p=0.01, Mann-Whitney U), (B) multipotent progenitor cells (MPPs, CD133+CD34+) (p>0.05, Mann-Whitney U), (C) erythro-myeloid restricted progenitors (EMPs, CD133-CD34+) (p=0.02 Mann-Whitney U), (D) hematopoietic and endothelial progenitor cells (HSCs/EPCs, CD133–CD34–) (p=0.01, Mann-Whitney U), and (E) endothelial progenitor cells (EPCs, CD133+CD34-) (p=0.03, Mann-Whitney U) in samples from 14 patients with myeloproliferative neoplasms and 5 control samples of cord blood. Relative c-MYC gene expressions were calculated with the formula 2-∆Ct = 2-(CTExample – CTReferance) applying ACTB as a reference gene. GraphPad Prism 8.0 software was used for the statistical analysis of gene expression data; in each figure, the standard deviation of the mean (SD) is shown with a bar; **p< 0.05.
Figure 2 Relative c-MYC gene expression analysis of (A) mononuclear cells (p=0.01, Mann-Whitney U), (B) multipotent progenitor cells (MPPs, CD133+CD34+) (p>0.05, Mann-Whitney U), (C) erythro-myeloid restricted progenitors (EMPs, CD133-CD34+) (p=0.02 Mann-Whitney U), (D) hematopoietic and endothelial progenitor cells (HSCs/EPCs, CD133–CD34–) (p=0.01, Mann-Whitney U), and (E) endothelial progenitor cells (EPCs, CD133+CD34-) (p=0.03, Mann-Whitney U) in samples from 14 patients with myeloproliferative neoplasms and 5 control samples of cord blood. Relative c-MYC gene expressions were calculated with the formula 2-∆Ct = 2-(CTExample – CTReferance) applying ACTB as a reference gene. GraphPad Prism 8.0 software was used for the statistical analysis of gene expression data; in each figure, the standard deviation of the mean (SD) is shown with a bar; **p< 0.05.CC BY-NC-ND · PubMed Central

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  1. Kelley, N., & Trebilcock, M. J. (1998). The making of the mosaic: A history of Canadian immigration policy. University of Toronto Press.

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Metadata

Authors
Uslu Bıçak, İ., Tokcan, B., Yavuz, A. S., & Tokdemir, S. S.
Venue
Turkish Journal of Hematology
Publisher
Galenos Yayinevi
Published
2023-03-03
Volume
40
Issue
1
Pages
28-36
ISSN
  • 1300-7777
  • 1308-5263
DOI
10.4274/tjh.galenos.2022.2022.0343
PMC
PMC9979741
Cited by
4
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CoChat. (2026). Citation check: Circulating CD133+/–CD34– Have Increased c-MYC Expression in Myeloproliferative Neoplasms. https://app.cochat.ai/papers/10.4274/tjh.galenos.2022.2022.0343
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Uslu Bıçak, İ., Tokcan, B., Yavuz, A. S., & Tokdemir, S. S. (2023). Circulating CD133+/–CD34– Have Increased c-MYC Expression in Myeloproliferative Neoplasms. Turkish Journal of Hematology. https://doi.org/10.4274/tjh.galenos.2022.2022.0343