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Cited by 116Essential Oils and Antimicrobial ActivityEnzyme Production and CharacterizationBacterial Genetics and Biotechnology

The DOI matches its Crossref record.

Eugenol Induced Inhibition of Extracellular Enzyme Production by Bacillus subtilis

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Thoroski, John, Blank, Greg, Biliaderis, Costas·1989·Journal of Food Protection
DOI 10.4315/0362-028x-52.6.399
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Abstract

Eugenol, the major essential oil of clove, in sublethal concentrations (0.02-0.03%, v/v) inhibited the production of alpha-amylase, protease, and subtilisin by Bacillus subtilis in laboratory media. Microscopic observations revealed that at these eugenol concentrations, B. subtilis cells appeared swollen and distorted and/or appeared as very long and thin filaments (> 100 μm). Of 20 amino acids investigated, only L-glutamic or L-aspartic acid (>5.0 mg/ml) prevented such morphogenic distortions when added to eugenol-containing media before inoculation. Addition of these amino acids also resulted in an increase in biomass and protease production. In contrast, the addition of serine (> 1.0 mg/ml) enhanced filamentous growth but reduced the production of protease and subtilisin.

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Metadata

Authors
Thoroski, J., Blank, G., & Biliaderis, C.
Affiliations
University of Manitoba (CA)
Venue
Journal of Food Protection
Publisher
Elsevier BV
Published
1989-06
Volume
52
Issue
6
Pages
399-403
ISSN
0362-028X (ISSN-L)
DOI
10.4315/0362-028x-52.6.399
OpenAlex
W2557933504
Cited by
116
References
0
Citations by year
2026: 1 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 11 · 2020: 7 · 2019: 7 · 2018: 9 · 2017: 6 · 2016: 10 · 2015: 4 · 2014: 4 · 2013: 8 · 2012: 5
FWCI
0.00
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Food Protection (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
eugenol, Bacillus subtilis, extracellular enzyme production, alpha-amylase, proteolytic enzymes, subtilisin, filamentous growth, essential oils, amino acid effects, glutamate, aspartic acid, serine, bacterial morphology

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