A comparison of the abilities of freshwater algae and bacteria to acquire and retain phosphorus1

Abstract

Extant physiological and in situ data provide contradictory answers to the question of whether the bacterioplankton can outcompete the phytoplankton for phosphorus at natural concentrations. Two phytoplankton species and three bacterial species isolated from the epilimnion of Lake Memphremagog, Québec, were grown in phosphorus‐limited continuous cultures and their abilities to take up and retain phosphorus compared. The algae showed orthophosphate uptake kinetics comparable to those reported elsewhere. In contrast, the specific orthophosphate uptake rates (i.e. uptake per unit cell P) by the bacteria were variable, but much higher than the algal rates at the low P concentrations characteristic of natural waters. Algal and bacterial alkaline phosphatase activities were similar and provided no evidence that the algae could utilize organic P more efficiently than the bacteria. The bacteria showed some tendency to excrete P more readily than the algae but mixed culture experiments indicated that excretion did little to alter the long term partitioning of phosphorus between the algae and the bacteria. The kinetic data suggest that planktonic bacteria are unlikely to be limited by phosphorus in situ. Furthermore, our results are consistent with the hypothesis that the bacteria should be markedly superior competitors at natural phosphate concentrations.