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Cited by 18Psoriasis: Treatment and PathogenesisEndoplasmic Reticulum Stress and DiseaseDermatology and Skin Diseases

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Endoplasmic reticulum stress links psoriasis vulgaris with keratinocyte inflammation

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Zhao, Min, Luo, Jinqiang, Xiao, Bin, Tang, Hongyan, Song, Fanjun, Ding, Xiaolei, Yang, Guilan·2020·Advances in Dermatology and Allergology
DOI 10.5114/ada.2020.93382PMC PMC7247056
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Abstract

INTRODUCTION: Endoplasmic reticulum stress (ERS) has been implicated in the pathogenesis of various inflammatory diseases. However, the role of ERS in psoriasis is still unclear. AIM: To examine ERS in psoriasis keratinocytes and to assess the association of ERS with skin inflammation response. MATERIAL AND METHODS: We investigated ERS in keratinocytes of normal skin, lesional and perilesional psoriasis vulgaris (PV) skin tissues using transmission electron microscope (TEM) examination, Western blot and immunostaining analysis. RESULTS: By TEM examination, we found that endoplasmic reticulum (ER) in psoriatic keratinocytes was ultrastructurally abnormal, with changes in ER morphology and the ER expansion. Using Western blot and immunostaining analysis, we showed that the expression of ERS-associated proteins, such as BiP, CHOP and XBP1, was enhanced in PV epidermis compared to the healthy skin. Moreover, abundant TNF-α protein was correlated to the increased BiP, CHOP and XBP1 expression in PV epidermis. CONCLUSIONS: Our findings demonstrate that PV keratinocytes have an increased ERS, which may contribute to the pathogenesis of PV.

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Crossref · 10.5114/ada.2020.93382

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Figures

Figure 1 Ultrastructural analysis of ER in PV skin. A1 – Control skin. The arrow demonstrates basal cells connected with spinous layer cells by desmosomes or hemidesmosomes. Nu – nucleus. A2 – Control skin. The arrow demonstrates ER. A large number of free ribosomes are observable in keratinocytes. B1 – Adjacent skin tissue. The arrows demonstrate metabolically active cells and basement membrane (BM). B2 – Adjacent skin tissue. Normal ER are observable in the keratinocytes. C1 – Psoriasis skin tissue. The arrow demonstrates diving cells. C2 – Psoriasis skin tissue. The arrow demonstrates inflated ER
Figure 1 Ultrastructural analysis of ER in PV skin. A1 – Control skin. The arrow demonstrates basal cells connected with spinous layer cells by desmosomes or hemidesmosomes. Nu – nucleus. A2 – Control skin. The arrow demonstrates ER. A large number of free ribosomes are observable in keratinocytes. B1 – Adjacent skin tissue. The arrows demonstrate metabolically active cells and basement membrane (BM). B2 – Adjacent skin tissue. Normal ER are observable in the keratinocytes. C1 – Psoriasis skin tissue. The arrow demonstrates diving cells. C2 – Psoriasis skin tissue. The arrow demonstrates inflated ERCC BY-NC-SA · PubMed Central
Figure 2 The expression of ERS-associated proteins is enhanced in psoriasis skin. A – Representative results of Western blot analysis. GADPH was used as a loading control. B – Quantitative results of protein expression basing on the intensity of Western blot assay as shown in panel A. Each dot represents one sample C – control normal skin, P – psoriasis A – adjacent tissue. ***P < 0.001, **p < 0.01, *p < 0.05.
Figure 2 The expression of ERS-associated proteins is enhanced in psoriasis skin. A – Representative results of Western blot analysis. GADPH was used as a loading control. B – Quantitative results of protein expression basing on the intensity of Western blot assay as shown in panel A. Each dot represents one sample C – control normal skin, P – psoriasis A – adjacent tissue. ***P < 0.001, **p < 0.01, *p < 0.05.CC BY-NC-SA · PubMed Central
Figure 3 BiP, XBP-1, CHOP and TNF-α are overexpressed by the PV epidermis. Sections of control normal skin (C), psoriasis vulgaris tissue (P) and adjacent skin tissue (A) were stained with anti- BiP, XBP-1, CHOP and TNF-α antibodies respectively and counterstained with haematoxylin. Representative images are shown. Scale bar, 100 μm
Figure 3 BiP, XBP-1, CHOP and TNF-α are overexpressed by the PV epidermis. Sections of control normal skin (C), psoriasis vulgaris tissue (P) and adjacent skin tissue (A) were stained with anti- BiP, XBP-1, CHOP and TNF-α antibodies respectively and counterstained with haematoxylin. Representative images are shown. Scale bar, 100 μmCC BY-NC-SA · PubMed Central

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Citations

Cites

Research works printed but not linked: 24

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Metadata

Authors
Zhao, M., Luo, J., Xiao, B., Tang, H., Song, F., Ding, X., & Yang, G.
Affiliations
  • Southern Medical University Shenzhen Hospital (CN)
  • University of Cologne (DE)
Corresponding author
Ding, Xiaolei
ORCID
Venue
Advances in Dermatology and Allergology
Publisher
Termedia Sp. z.o.o.
Published
2020
Volume
37
Issue
1
Pages
34-40
ISSN
  • 0860-6218 (ISSN-L)
  • 1642-395X
DOI
10.5114/ada.2020.93382
PMC
PMC7247056
OpenAlex
W3013262169
Cited by
18
References
1
Citations by year
2026: 6 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 1
FWCI
0.53
Type
journal article
Language
en
Open access
Locations
  • Advances in Dermatology and Allergology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Kölner Universitäts PublikationsServer (Universität zu Köln) (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Chinese Academy of Sciences
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Endoplasmic Reticulum Stress and Disease
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
Keywords
endoplasmic reticulum stress, plaque psoriasis, keratinocytes, skin inflammation, BiP, CHOP, XBP1, TNF-α, endoplasmic reticulum ultrastructure, perilesional skin, psoriatic epidermis

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