jungsukimlab/NucleiIsolation: Comparative analysis of nuclei isolation methods for brain single-nucleus RNA sequencing
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Contains code used in the manuscript "Comparative analysis of nuclei isolation methods for brain single-nucleus RNA sequencing"
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The machine-assisted nuclei isolation protocol yields high-quality nuclei with extremely low contamination (median mitochondrial reads <0.5%, median ribosomal reads <0.03%) across all cell types.
“To acquire exceptionally clean snRNA‐seq data, we established a machine‐assisted nuclei isolation protocol that has been shown to yield high‐quality nuclei with extremely low contamination (median mitochondrial reads < 0.5%, median ribosomal reads < 0.03%) across all cell types. 22”
From Unique transcriptomic alterations in 5XFAD;PS19 mouse model identify glial lipid dysregulation and coordinated microglial–oligodendrocyte responses (2026) · cites this paper as [22]
The machine-assisted nuclei isolation protocol minimizes ambient RNA contamination and enables capture of large numbers of high-quality nuclei across diverse cell types.
“Our machine‐assisted nuclei isolation protocol minimizes ambient RNA contamination 22 and enables the capture of large numbers of high‐quality nuclei across diverse cell types.”
From Unique transcriptomic alterations in 5XFAD;PS19 mouse model identify glial lipid dysregulation and coordinated microglial–oligodendrocyte responses (2026) · cites this paper as [22]
- Authors
- Kersey, H., & Acri, D.
- Affiliations
- National Institute on Aging
- ORCID
- Holly Kersey
- Venue
- Zenodo
- Publisher
- Zenodo
- Published
- 2026-01-26
- OpenAlex
- W7125700841
- Cited by
- 0
- References
- 0
- Type
- software
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- Zenodo (CERN European Organization for Nuclear Research) (cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- nuclei isolation, single-nucleus RNA sequencing, brain, method comparison, single-nucleus transcriptomics
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