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Studies on a Neutral Exopolysaccharide of Lactobacillus fermentum TDS030603
Open accessAbstract
A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6 substituted galactopyranose and O-2,3 disubstituted glucopyranose. The minor saccharide consisted of non-reducing galactopyranose, O-3 substituted glucopyranose, O-6 substituted galactopyranose and O-2,3 disubstituted glucopyranose. The results suggest that these exopolysaccharides are novel saccharides that differ from previously reported products of Lactobacillus fermentum strains. Their presence may contribute to the ropy properties of the medium.
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“Likewise, Lim. fermentum F6, Lim. fermentum TDS030603 and Lcb. casei CRL 870 produced elevated amounts of EPS when incubated at 37 °C [64,69].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [69]
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Cited by
Sorted by citations
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194
Naghmouchi, K., Belguesmia, Y., Bendali, F., Spano, G., Seal, B. S., & Drider, D. (2019) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2019.1688250 · Cited by 150
Exopolysaccharides of Lactic Acid Bacteria for Food and Colon Health Applications
TSUDA, H. (2013) · InTech eBooks · doi:10.5772/50839 · Cited by 76
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Lactobacillus fermentum: Could EPS production ability be responsible for functional properties?
Ale, E. C., Rojas, M. F., Reinheimer, J. A., & Binetti, A. G. (2020) · Food Microbiology · doi:10.1016/j.fm.2020.103465 · Cited by 61
Metadata
- Authors
- Leo, F., Hashida, S., Kumagai, D., Uchida, K., Motoshima, H., Arai, I., Asakuma, S., Fukuda, K., & Urashima, T.
- Affiliations
- Obihiro University of Agriculture and Veterinary Medicine (JP)
- Yotsuba MILK Products Co., Ltd. (Japan) (JP)
- Hokkaido Agricultural Research Center (JP)
- Corresponding author
- Urashima, Tadasu
- Venue
- Journal of Applied Glycoscience
- Published
- 2007
- Volume
- 54
- Issue
- 4
- Pages
- 223-229
- ISSN
- 1344-7882 (ISSN-L)
- 1880-7291
- OpenAlex
- W2048517327
- Cited by
- 22
- References
- 28
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 3 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2
- FWCI
- 1.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Applied Glycoscience (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Funders
- Ministry of Economy, Trade and Industry
- Agency for Natural Resources and Energy
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- Lactobacillus fermentum, polysaccharide structure, galactose, D-glucose, tetrasaccharide repeating unit, glycosylation patterns, MRS broth, production optimization, bacterial supernatant
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