Structure-activity relationships of the intramolecular disulfide bonds in coprisin, a defensin from the dung beetle
Open accessAbstract
Defensins, which are small cationic molecules produced by organisms as part of their innate immune response, share a common structural scaffold that is stabilized by three disulfide bridges. Coprisin is a 43-amino acid defensin-like peptide from Copris tripartitus. Here, we report the intramolecular disulfide connectivity of cysteine-rich coprisin, and show that it is the same as in other insect defensins. The disulfide bond pairings of coprisin were determined by combining the enzymatic cleavage and mass analysis. We found that the loss of any single disulfide bond in coprisin eliminated all antibacterial, but not antifungal, activity. Circular dichroism (CD) analysis showed that two disulfide bonds, Cys20-Cys39 and Cys24-Cys41, stabilize coprisin's α-helical region. Moreover, a BLAST search against UniProtKB database revealed that coprisin's α-helical region is highly homologous to those of other insect defensins.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Coprisin, a defensin-like 43-mer peptide with three disulfide bonds, has been suggested to be a crucial participant in reactive oxygen species, especially hydroxyl radicals.
“Coprisin, a defensin-like 43-mer peptide with three disulfide bonds [187], has been suggested to be a crucial participant in reactive oxygen species (ROS), especially hydroxyl radicals (•OH) [188].”
From The Role of Reactive Species on Innate Immunity (2022) · cites this paper as [187]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 19 · Cited by 25
- Authors
- Lee, J., Lee, D., Choi, H., Kim, H. H., Kim, H., Hwang, J. S., Lee, D. G., & Kim, J. I.
- Affiliations
- Kyungpook National University (KR)
- Daejin University (KR)
- Gwangju Institute of Science and Technology (KR)
- Corresponding author
- Kim, Jae Il
- Venue
- BMB Reports
- Published
- 2014-11-30
- Volume
- 47
- Issue
- 11
- Pages
- 625-630
- ISSN
- 1976-6696 (ISSN-L)
- PMC
- PMC4281341
- OpenAlex
- W2037497267
- Cited by
- 25
- References
- 19
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 5 · 2020: 1 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 1
- FWCI
- 1.90
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- BMB Reports (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- National Research Foundation
- Rural Development Administration (PJ008158, NRF-C1ABA001-2011-0018559)
- National Research Foundation of Korea
- Ministry of Education, Science and Technology (2013R1A1A2009798)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- defensins, intramolecular disulfide bond, disulfide connectivity, Copris tripartitus, dung beetles, antibacterial activity, antifungal activity, alpha-helix stabilization, innate immunity, insect peptides, structure-activity relationship
- MeSH terms
- Amino Acid Sequence, Animals, Anti-Bacterial Agents, Antifungal Agents, Coleoptera, Circular Dichroism, Cysteine, Disulfides, Fungi, Gram-Negative Bacteria, Gram-Positive Bacteria, Microbial Sensitivity Tests, Molecular Sequence Data, Structure-Activity Relationship, Sequence Alignment, Protein Structure, Secondary, Insect Proteins, Catalytic Domain
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
