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Cited by 32Seaweed-derived Bioactive CompoundsAlgal biology and biofuel productionMicrobial Metabolism and Applications

Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis

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School of Pharmacy, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia, Julianti, Elin, Susanti, Susanti, School of Pharmacy, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia, Singgih, Marlia, School of Pharmacy, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung 40132, Indonesia, Mulyani, Laida Neti, Department of Pharmacy, Faculty of Mathematics and Sciences, Sriwijaya University, Jl. Palembang-Indralaya KM 32, Ogan Ilir, South Sumatra, Indonesia·2019·Journal of Mathematical and Fundamental Sciences
DOI 10.5614/j.math.fund.sci.2019.51.2.6
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Abstract

Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.

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  • These compounds are also used as natural coloring for food and cosmetics, which are carotenoids, chlorophyll and phycobiliprotein.

    “These compounds are also used as natural coloring for food and cosmetics, which are carotenoids, chlorophyll and phycobiliprotein [58].”

    From SPIRULINA (ARTHROSPIRA PLATENSIS): AN ALTERNATIVE SOURCE OF NUTRIENTS - A REVIEW (2025) · cites this paper as [58]

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Authors
School of Pharmacy, I. T. B., Julianti, E., Susanti, S., School of Pharmacy, I. T. B., Singgih, M., School of Pharmacy, I. T. B., Mulyani, L. N., & Department of Pharmacy, F. O. M. A. S.
Affiliations
  • Bandung Institute of Technology (ID)
  • Sriwijaya University (ID)
ORCID
Venue
Journal of Mathematical and Fundamental Sciences
Publisher
The Institute for Research and Community Services (LPPM) ITB
Published
2019-08
Volume
51
Issue
2
Pages
168-176
ISSN
  • 1978-3043 (ISSN-L)
  • 2337-5760
  • 2338-5510
DOI
10.5614/j.math.fund.sci.2019.51.2.6
OpenAlex
W2967993260
Cited by
32
References
2
Citations by year
2026: 1 · 2025: 8 · 2024: 7 · 2023: 5 · 2022: 5 · 2021: 4 · 2020: 2
FWCI
2.45
Type
journal article
Language
en
Open access
Licence
cc-by-nd
Locations
  • Journal of Mathematical and Fundamental Sciences (publishedVersion, cc-by-nd)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Kementerian Riset, Teknologi dan Pendidikan Tinggi
  • Direktorat Jenderal Pendidikan Tinggi
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
Keywords
phycocyanin, Spirulina platensis, pigment extraction, freeze-thaw extraction, ammonium sulfate precipitation, size exclusion chromatography, microalgal biomass, pigment purification, molecular weight

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