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Cited by 349

The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.

Dehydrogenase Activity in the Soil Environment

Open access
Wolinska, Agnieszka, Stepniewsk, Zofia·2012·Dehydrogenases

Abstract

The most common laboratory procedure used for DHA determination is the method developed by Casida et al. (1964). According this method, specific dyes such as the triphenyltetrazolium chloride (TTC), that can specify the flow of electrons are useful indicators of electron transport system (ETS) activity. By the reduction of colorless, water soluble substrate (TTC) by dehydrogenases present in the soil environment, an insoluble product with red color (triphenylformazan-TPF) is formed. TPF can be easily quantified calorimetrically at the range of visible light (485 nm). This test however, reflected positive answer only at neutral range of pH and in presence of calcium carbonate for buffering soil system. Briefly, if the red colors of soil samples prepared for spectrophotometer analyses are more intensive, the measured level of DHA is higher. Consequently, soil samples without red colors or those with light red colors are characterized by lower DHA values.

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Metadata

Authors
Wolinska, A., & Stepniewsk, Z.
Venue
Dehydrogenases
Publisher
InTech
Published
2012-11-14
OpenAlex
W1570233343
Cited by
349
References
41
FWCI
15.56
Type
book chapter
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Dehydrogenase Activity in the Soil Environment. https://app.cochat.ai/papers/10.5772/48294
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Wolinska, A., & Stepniewsk, Z. (2012). Dehydrogenase Activity in the Soil Environment. Dehydrogenases. https://doi.org/10.5772/48294

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