The CRISPR/Cas9 System for Crop Improvement: Progress and Prospects
Open accessAbstract
The global demand for high-quality crops is continuously growing with time. Crop improvement techniques have a long history and they had been applied since the beginning of domestication of the first agricultural plants. Since then, various new techniques have and are being developed to further increase the commercial value and yield of crops. The latest crop improvement technique known as genome editing is a technique that enables precise modification of the plant genome via knocking out undesirable genes or enabling genes to gain new function. The variants generated from the genome editing are indistinguishable from naturally occurring variation. It is also less time-consuming and more readily accepted in the market commercially. The usage of genome editing has proven to be advantages and plays a promising role in future crop improvement efforts. Therefore, in this chapter, we aim to highlight the progress and application of genome editing techniques, in particular, the CRISPR/Cas9 system as a powerful genome editing tool for crop improvement. In addition, the challenges and future prospects of this technology for crop improvement will also be discussed.
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Compared to other gene-editing methods such as TALEN or zinc finger nuclease (ZFN), CRISPR/Cas9 has advantages in simple target design, multiplexed mutation, versatility, and efficiency.
“Compared to other gene-editing methods such as TALEN or zinc finger nuclease (ZFN), CRISPR/Cas9 has advantages in simple target design, multiplexed mutation, versatility, and efficiency (Leong et al., 2018).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Leong et al., 2018
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References 69 · Cited by 5
Research works printed but not linked: 3
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- Authors
- Leong, K.-Y. B., Chan, Y.-H., Abdullah, W. M. A. N. W., Lim, S.-H. E., & Lai, K.-S.
- Affiliations
- Universiti Tunku Abdul Rahman (MY)
- Universiti Putra Malaysia (MY)
- Higher Colleges of Technology (AE)
- Corresponding author
- Lai, Kok-Song
- Venue
- Next Generation Plant Breeding
- Publisher
- InTech
- Published
- 2018-09-26
- OpenAlex
- W2895535274
- Cited by
- 5
- References
- 69
- Citations by year
- 2026: 2 · 2023: 1 · 2022: 1 · 2021: 1
- FWCI
- 0.34
- Type
- book chapter
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- InTech eBooks (publishedVersion, cc-by)
- BiblioBoard Library Catalog (Open Research Library) (submittedVersion, cc-by)
- IntechOpen (submittedVersion, cc-by)
- Funders
- Ministry of Higher Education, Malaysia (FRGS/1/)
- Universiti Putra Malaysia (GP-IPM/2015/9450800)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › General Agricultural and Biological Sciences › Agriculture and Biological Studies
- Keywords
- CRISPR/Cas9, genome editing, crop improvement, plant genome modification, gene knockout, gene activation, agricultural yields
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