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Investigations of antioxidant and antibacterial activity of leaf extracts of Azadirachta indica
Open accessAbstract
Active ingredients of medicinal plants have been used to cure several human diseases. Azadirachta indica is one of the most versatile medicinal plants having a wide spectrum of biological activity due to the presence of large number of bioactive compounds. The present study was conducted to evaluate antimicrobial and antioxidant efficiency and phytochemical screening of A. indica leaf extract using methanol as a solvent. A qualitative phytochemical screening was performed for the detection of various phytochemicals. Then, the quantitative determination of total phenols, flavonoids and proanthocyanidins was done and expressed in terms of gallic acid and rutin equivalent. Total phenolic, flavonoid and proanthocyanidin content were found to be 85.9±4.0, 104.9±5.5 and 65.4±13.9 mg/g of plant extract, respectively. Also, the antibacterial activity was performed using six different bacterial strains: Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Salmonella typhi (ATCC 14028), Klebsiella pneumoniae (ATCC 700603), Pseudomonas aeruginosa (ATCC 27853) and Proteus vulgaris (ATCC 35659). It was found that the maximum zone of inhibition of 22±3 mm was shown against S. aureus using 700 µg plant extract. Similarly, the antioxidant activity of the methanolic extract of plant was also determined and it was found that maximum inhibition obtained was 71.23% when 500 µg plants extract was used. Key words: Azadirachta indica, methanol, antibacterial, antioxidant.
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“Phytochemical components of neem leaf aqueous and methanol extracts have been reported by the researchers (Dash et al., 2017;Bharat et al., 2015) are in close agreement to our current study.”
From Application of Azadirachta indica (Neem) organic crude macerated extracts against postharvest decay of fruits caused by Penicillium expansum, Colletotrichum gloeosporioides and Botrytis cinerea (2025) · cites this paper as Bharat et al., 2015
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ALI, J., HUSSAIN, A., IKRAM, M., SIDDIQUE, M., ZAHOOR, M., ULLAH, R., IBRAHIM, M. A., GULFAM, N., & SHAH, A. B. (2025) · Notulae Botanicae Horti Agrobotanici Cluj-Napoca · doi:10.15835/nbha53114086 · Cited by 3
Metadata
- Authors
- Bharat, P., Sagar, R., Sulav, R., & Ankit, P.
- Affiliations
- Pokhara University (NP)
- Corresponding author
- Bharat, Pokhrel
- Venue
- African Journal of Biotechnology
- Publisher
- Academic Journals
- Published
- 2015-11-18
- Volume
- 14
- Issue
- 46
- Pages
- 3159-3163
- ISSN
- 1684-5315 (ISSN-L)
- OpenAlex
- W2183477265
- Cited by
- 38
- References
- 0
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 6 · 2023: 11 · 2022: 8 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 1
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by
- Locations
- AFRICAN JOURNAL OF BIOTECHNOLOGY (publishedVersion, cc-by)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- Azadirachta indica, neem leaf extract, antibacterial activity, antioxidant activity, phytochemical screening, total phenolic content, flavonoids, proanthocyanidins, methanol extract, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Proteus vulgaris
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- Bharat, P., Sagar, R., Sulav, R., & Ankit, P. (2015). Investigations of antioxidant and antibacterial activity of leaf extracts of Azadirachta indica. African Journal of Biotechnology. https://doi.org/10.5897/ajb2015.14811
