Bacillus subtilis NCIM2063 batch cultivation: The influence of the substrate concentration and oxygen transfer rate on the biomass yield
Open accessAbstract
The objective of this study was to investigate the growth kinetics of Bacillus subtilis NCIM 2063 under different cultivation conditions. For that purpose, the growth medium was supplemented with different glucose concentrations and exposed to different values of the oxygen transfer rate (OTR). As a result, it was found that the biomass yield, the specific growth rate and generation time were affected by the change of cultivation conditions and the composition of the nutrient medium. It was confirmed that glucose is readily available substrate that enhances the cultivation in the concentration range of 5-15 g/L. Also, it was proved that the restriction of available oxygen lowers the biomass yield and slows down the replication of bacteria. The maximum biomass concentration was achieved in the medium with 10 g/L of glucose and the OTR value of 10 mol/m³ h, when the specific growth rate and generation time were 0.273 h-1 and 2.54 h, respectively. Such biomass yield can be achieved in only 24 h, which is also economically acceptable. Therefore, the growth conditions optimized in this experiment are recommended as optimized values for further research, and can be used for economically sustainable and efficient cultivation of B. subtilis NCIM 2063.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Glucose is a preferred carbon source for B. subtilis and other Bacilli.
“Previous studies also show that glucose is a preferred carbon source for B. subtilis and other Bacilli [32-34].”
From Optimization of Bacillus subtilis PW12 biomass production using RSM: a preliminary study towards single-cell protein production for aquaculture (2024) · cites this paper as [33]
Stamenkovic et al. [33] reported that biomass production of B. subtilis NCIM2063 could be enhanced when the growth medium was supplemented with 10 g/L glucose.
“Stamenkovic et al. [33] reported that biomass production of B.subtilis NCIM2063 could be enhanced when the growth medium was supplemented with 10 g/L of glucose.”
From Optimization of Bacillus subtilis PW12 biomass production using RSM: a preliminary study towards single-cell protein production for aquaculture (2024) · cites this paper as [33]
Studies by Nguyen [22], Stamenkovic et al. [33], Naveed et al. [36], and Ghasemi and Ahmadzadeh [54] reported biomass yields of 3.033 g/L, 6 g/L, 3.81 g/L, and 0.5 g/L, respectively, for different B. subtilis strains.
“Studies of Nguyen [22], Stamenkovic et al. [33], Naveed et al. [36], and Ghasemi and Ahmadzadeh [54] reported biomass yields as 3.033 g/L, 6 g/L, 3.81 g/L, and 0.5 g/L, respectively, for different B. subtilis strains.”
From Optimization of Bacillus subtilis PW12 biomass production using RSM: a preliminary study towards single-cell protein production for aquaculture (2024) · cites this paper as [33]
Cites
A Procedure for High-Yield Spore Production by Bacillus s ubtilis
Monteiro, S. M., Clemente, J. J., Henriques, A. O., Gomes, R. J., Carrondo, M. J., & Cunha, A. E. (2008) · Biotechnology Progress · doi:10.1021/bp050062z · Cited by 119 · see citing works
Bacillus Based Microbial Formulations: Optimization of the Production Process
Stamenkovic-Stojanovic, S., Karabegovic, I., Beskoski, V., Nikolic, N., & Lazic, M. (2019) · Hemijska industrija · doi:10.2298/hemind190214014s · Cited by 37 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Gracious, M., & Nandakumar, S. (2024) · Journal of Applied Biology & Biotechnology · doi:10.7324/jabb.2024.177031 · Cited by 2 · see citing works
“Stamenkovic et al. [33] reported that biomass production of B.subtilis NCIM2063 could be enhanced when the growth medium was supplemented with 10 g/L of glucose.”
- Authors
- Stamenković-Stojanović, S., Karabegović, I., Beškoski, V., Nikolić, N., & Lazić, M.
- Affiliations
- University of Nis (RS)
- Venue
- Advanced Technologies
- Published
- 2020
- Volume
- 9
- Issue
- 1
- Pages
- 44-49
- ISSN
- 2217-9712 (ISSN-L)
- 2406-2979
- 2406-3037
- OpenAlex
- W3042777547
- Cited by
- 5
- References
- 2
- Citations by year
- 2026: 2 · 2025: 1 · 2022: 2
- FWCI
- 0.16
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Advanced technologies (publishedVersion)
- SCIndeks (publishedVersion, other-oa)
- Funders
- Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja (43004, III 43004, 451-03-68/, 451-03-68/2020–14/200133, 451-03-68 / 2020-14)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Keywords
- batch culture, glucose concentration, oxygen transfer rate, biomass yield, growth kinetics, specific growth rate, generation time, oxygen limitation, fermentation optimization
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
