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Cited by 110Bee Products Chemical AnalysisHealthcare and Venom ResearchWound Healing and Treatments

Propolis Standardized Extract (EPP-AF®), an Innovative Chemically and Biologically Reproducible Pharmaceutical Compound for Treating Wounds

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Berretta, Andresa Aparecida, Nascimento, Andresa Piacezzi, Bueno, Paula Carolina Pires, de Oliveira Lima Leite Vaz, Mirela Mara, Marchetti, Juliana Maldonado·2012·International Journal of Biological Sciences
DOI 10.7150/ijbs.3641PMC PMC3314192
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Abstract

The aim of this study was to develop a formulation, containing the propolis standardized extract (EPP-AF(®)), which can assist in the healing of skin lesions. To achieve this objective the antimicrobial activity and chemical composition of the propolis extract was determined. The final product was subjected to in vitro and in vivo pre-clinical evaluation. The broth macrodilution method was used to determine the antimicrobial activity of the extracts and formulations against the microorganisms most commonly found in burns, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis. Wistar rats with puncture wounded skin were used to evaluate the wound healing properties of propolis. The results of chemical and biological characterization demonstrated the batch-to-batch reproducibility of the standardized extract which is an unprecedented result. The antimicrobial and wound healing activity of the pharmaceutical studied showed the best results when samples contain 3.6% propolis, suggesting that this is the most promising composition.

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Authors
Berretta, A. A., Nascimento, A. P., Bueno, P. C. P., de Oliveira Lima Leite Vaz, M. M., & Marchetti, J. M.
Affiliations
  • Universidade de São Paulo (BR)
  • Apis Flora (Brazil) (BR)
  • Universidade Estadual Paulista (Unesp) (BR)
Corresponding author
Berretta, Andresa Aparecida
ORCID
Venue
International Journal of Biological Sciences
Publisher
Ivyspring International Publisher
Published
2012
Volume
8
Issue
4
Pages
512-521
ISSN
1449-2288 (ISSN-L)
DOI
10.7150/ijbs.3641
PMC
PMC3314192
OpenAlex
W2145579888
Cited by
110
References
1
Citations by year
2026: 4 · 2025: 8 · 2024: 6 · 2023: 16 · 2022: 15 · 2021: 11 · 2020: 6 · 2019: 8 · 2018: 3 · 2017: 8 · 2016: 6 · 2015: 3 · 2014: 2 · 2013: 12 · 2012: 2
FWCI
5.86
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • International Journal of Biological Sciences (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (publishedVersion)
  • Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Bee Products Chemical Analysis
  • Health Sciences › Medicine › Pharmacology › Healthcare and Venom Research
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
Keywords
wound healing, antimicrobial activity, skin lesions, burns, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, batch-to-batch reproducibility, drug formulation, puncture wounds
MeSH terms
Animals, Anti-Infective Agents, Chromatography, High Pressure Liquid, Escherichia coli, Male, Microbial Sensitivity Tests, Propolis, Pseudomonas aeruginosa, Staphylococcus aureus, Wound Healing, Rats, Wistar, Rats

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