CoChat
Sign Up
Cited by 29Barrier Structure and Function StudiesAngiogenesis and VEGF in CancerProtein Kinase Regulation and GTPase Signaling

GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury.

Open access
Chen, Dan-Yang, Sun, Ning-He, Lu, Ya-Ping, Hong, Ling-Juan, Cui, Tian-Tian, Wang, Cheng-Kun, Chen, Xing-Hui, Wang, Shuai-Shuai +6 more·2019·Theranostics
DOI 10.7150/thno.34168PMC PMC6735362
Open this paper in CoChat — free account

Abstract

: Taken together, our results provide the first evidence for a role of GPR124 in pericyte migration under ischemic conditions and suggest that GPR124 was essential for Cdc42 activation and filopodia formation.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.7150/thno.34168

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Orphan receptor GPR124 is differently expressed in FXS and control astrocytes and may represent a partner receptor that acts through WNT/β-catenin signaling, which is altered in FXS models.

    “Orphan receptor GPR124 (Chen et al., 2019) is differently expressed in FXS and control astrocytes and may represent a partner receptor that acts through WNT/β-catenin signaling, which is altered in FXS models (Peteri et al., 2021).”

    From Astrocytes in fragile X syndrome (2024) · cites this paper as Chen et al., 2019

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 64

  1. [1]Kisler K Nelson AR Montagne A Zlokovic BV Cerebral blood flow regulation and neurovascular dysfunction in Alzheimer disease Nat Rev Neurosci 2017 18 419 34 28515434 PMC5759779
  2. [2]Lu YM Tao RR Huang JY Li LT Liao MH Li XM P2X7 signaling promotes microsphere embolism-triggered microglia activation by maintaining elevation of Fas ligand J Neuroinflammation 2012 9 172 22789015 PMC3420259
  3. [3]Han F Tao RR Zhang GS Lu YM Liu LL Chen YX Melatonin ameliorates ischemic-like injury-evoked nitrosative stress: Involvement of HtrA2/PED pathways in endothelial cells J Pineal Res 2011 50 281 91 21198825
  4. [4]Haitina T Olsson F Stephansson O Alsio J Roman E Ebendal T Expression profile of the entire family of Adhesion G protein-coupled receptors in mouse and rat BMC Neurosci 2008 9 43 18445277 PMC2386866
  5. [5]Lagerstrom MC Schioth HB Structural diversity of G protein-coupled receptors and significance for drug discovery Nat Rev Drug Discov 2008 7 339 57 18382464
  6. [6]Yona S Lin HH Siu WO Gordon S Stacey M Adhesion-GPCRs: emerging roles for novel receptors Trends Biochem Sci 2008 33 491 500 18789697
  7. [7]Cullen M Elzarrad MK Seaman S Zudaire E Stevens J Yang MY GPR124, an orphan G protein-coupled receptor, is required for CNS-specific vascularization and establishment of the blood-brain barrier Proc Natl Acad Sci U S A 2011 108 5759 64 21421844 PMC3078373
  8. [8]Dejana E Nyqvist D News from the brain: the GPR124 orphan receptor directs brain-specific angiogenesis Sci Transl Med 2010 2 58ps3 21084718
  9. [10]Anderson KD Pan L Yang XM Hughes VC Walls JR Dominguez MG Angiogenic sprouting into neural tissue requires Gpr124, an orphan G protein-coupled receptor Proc Natl Acad Sci U S A 2011 108 2807 12 21282641 PMC3041062
  10. [11]Vanhollebeke B Stone OA Bostaille N Cho C Zhou Y Maquet E Tip cell-specific requirement for an atypical Gpr124- and Reck-dependent Wnt/beta-catenin pathway during brain angiogenesis Elife 2015 4 PMC4456509 26051822
  11. [12]Chang J Mancuso MR Maier C Liang X Yuki K Yang L Gpr124 is essential for blood-brain barrier integrity in central nervous system disease Nat Med 2017 23 450 60 28288111 PMC5559385
  12. [13]Gong DM Zhang YL Chen DY Hong LJ Han F Liu QB Endothelial GPR124 Exaggerates the Pathogenesis of Atherosclerosis by Activating Inflammation Cell Physiol Biochem 2018 45 547 57 29402834
  13. [14]Calderon-Zamora L Ruiz-Hernandez A Romero-Nava R Leon-Sicairos N Canizalez-Roman A Hong E Possible involvement of orphan receptors GPR88 and GPR124 in the development of hypertension in spontaneously hypertensive rat Clin Exp Hypertens 2017 39 513 9 28678544
  14. [15]Kaur H Carvalho J Looso M Singh P Chennupati R Preussner J Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system Nat Commun 2017 8 15700 28621310 PMC5481776
  15. [16]Zhou Y Nathans J Gpr124 controls CNS angiogenesis and blood-brain barrier integrity by promoting ligand-specific canonical wnt signaling Dev Cell 2014 31 248 56 25373781 PMC4223636
  16. [17]Posokhova E Shukla A Seaman S Volate S Hilton MB Wu B GPR124 functions as a WNT7-specific coactivator of canonical beta-catenin signaling Cell Rep 2015 10 123 30 25558062 PMC4331012
  17. [18]Cho C Smallwood PM Nathans J Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-Specific Signaling in Mammalian CNS Angiogenesis and Blood-Brain Barrier Regulation Neuron 2017 95 1221 5 28858622
  18. [19]Kelly-Goss MR Sweat RS Stapor PC Peirce SM Murfee WL Targeting pericytes for angiogenic therapies Microcirculation 2014 21 345 57 24267154 PMC4079092
  19. [20]Park DY Lee J Kim J Kim K Hong S Han S Plastic roles of pericytes in the blood-retinal barrier Nat Commun 2017 8 15296 28508859 PMC5440855
  20. [21]Teichert M Milde L Holm A Stanicek L Gengenbacher N Savant S Pericyte-expressed Tie2 controls angiogenesis and vessel maturation Nat Commun 2017 8 16106 28719590 PMC5520106
  21. [22]Armulik A Genove G Betsholtz C Pericytes: developmental, physiological, and pathological perspectives, problems, and promises Dev Cell 2011 21 193 215 21839917
  22. [23]Winkler EA Bell RD Zlokovic BV Central nervous system pericytes in health and disease Nat Neurosci 2011 14 1398 405 22030551 PMC4020628
  23. [24]Hall CN Reynell C Gesslein B Hamilton NB Mishra A Sutherland BA Capillary pericytes regulate cerebral blood flow in health and disease Nature 2014 508 55 60 24670647 PMC3976267
  24. [25]Fernandez-Klett F Potas JR Hilpert D Blazej K Radke J Huck J Early loss of pericytes and perivascular stromal cell-induced scar formation after stroke J Cereb Blood Flow Metab 2013 33 428 39 23250106 PMC3587816
  25. [26]Hamilton NB Attwell D Hall CN Pericyte-mediated regulation of capillary diameter: a component of neurovascular coupling in health and disease Front Neuroenergetics 2010 2 PMC2912025 20725515
  26. [27]Yemisci M Gursoy-Ozdemir Y Vural A Can A Topalkara K Dalkara T Pericyte contraction induced by oxidative-nitrative stress impairs capillary reflow despite successful opening of an occluded cerebral artery Nat Med 2009 15 1031 7 19718040
  27. [28]Han F Chen YX Lu YM Huang JY Zhang GS Tao RR Regulation of the ischemia-induced autophagy-lysosome processes by nitrosative stress in endothelial cells J Pineal Res 2011 51 124 35 21392095
  28. [29]Crouch EE Doetsch F FACS isolation of endothelial cells and pericytes from mouse brain microregions Nat Protoc 2018 13 738 51 29565899
  29. [30]Kuo JC Han X Hsiao CT Yates JR 3rd Waterman CM Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for beta-Pix in negative regulation of focal adhesion maturation Nat Cell Biol 2011 13 383 93 21423176 PMC3279191
  30. [31]Tan C Lu NN Wang CK Chen DY Sun NH Lyu H Endothelium-Derived Semaphorin 3G Regulates Hippocampal Synaptic Structure and Plasticity via Neuropilin-2/PlexinA4 Neuron 2019 101 920 37 e13 30685224
  31. [32]Wang CK Cheng J Liang XG Tan C Jiang Q Hu YZ A H2O2-Responsive Theranostic Probe for Endothelial Injury Imaging and Protection Theranostics 2017 7 3803 13 29109778 PMC5667350
  32. [33]Zhang J Carver CM Choveau FS Shapiro MS Clustering and Functional Coupling of Diverse Ion Channels and Signaling Proteins Revealed by Super-resolution STORM Microscopy in Neurons Neuron 2016 92 461 78 27693258 PMC5553284
  33. [34]Tao RR Wang H Hong LJ Huang JY Lu YM Liao MH Nitrosative stress induces peroxiredoxin 1 ubiquitination during ischemic insult via E6AP activation in endothelial cells both in vitro and in vivo Antioxid Redox Signal 2014 21 1 16 24295341 PMC4048580
  34. [35]Gregor M Osmanagic-Myers S Burgstaller G Wolfram M Fischer I Walko G Mechanosensing through focal adhesion-anchored intermediate filaments FASEB J 2014 28 715 29 24347609
  35. [36]De Pascalis C Etienne-Manneville S Single and collective cell migration: the mechanics of adhesions Mol Biol Cell 2017 28 1833 46 28684609 PMC5541834
  36. [37]Geiger B Spatz JP Bershadsky AD Environmental sensing through focal adhesions Nat Rev Mol Cell Biol 2009 10 21 33 19197329
  37. [38]Han MKL de Rooij J Converging and Unique Mechanisms of Mechanotransduction at Adhesion Sites Trends Cell Biol 2016 26 612 23 27036655
  38. [39]Tao RR Ji YL Lu YM Fukunaga K Han F Targeting nitrosative stress for neurovascular protection: new implications in brain diseases Curr Drug Targets 2012 13 272 84 22023405
  39. [40]Cheng JPX Nichols BJ Caveolae: One Function or Many? Trends Cell Biol 2016 26 177 89 26653791
  40. [41]Li X Tao RR Hong LJ Cheng J Jiang Q Lu YM Visualizing peroxynitrite fluxes in endothelial cells reveals the dynamic progression of brain vascular injury J Am Chem Soc 2015 137 12296 303 26352914
  41. [42]Deakin NO Turner CE Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration J Cell Biol 2014 206 395 413 25070956 PMC4121979
  42. [43]Goldstein B Macara IG The PAR proteins: fundamental players in animal cell polarization Dev Cell 2007 13 609 22 17981131 PMC2964935
  43. [44]Ranganathan P Mohamed R Jayakumar C Ramesh G Guidance cue netrin-1 and the regulation of inflammation in acute and chronic kidney disease Mediators Inflamm 2014 2014 525891 24991088 PMC4065723
  44. [45]Gangaraju S Sultan K Whitehead SN Nilchi L Slinn J Li X Cerebral endothelial expression of Robo1 affects brain infiltration of polymorphonuclear neutrophils during mouse stroke recovery Neurobiol Dis 2013 54 24 31 23473743
  45. [46]Cramer LP Kay RR Zatulovskiy E Repellent and Attractant Guidance Cues Initiate Cell Migration by Distinct Rear-Driven and Front-Driven Cytoskeletal Mechanisms Curr Biol 2018 28 995 1004 e3 29526589 PMC5863766
  46. [47]Melton AC Yee HF Hepatic stellate cell protrusions couple platelet-derived growth factor-BB to chemotaxis Hepatology 2007 45 1446 53 17465006
  47. [48]Paluch EK Aspalter IM Sixt M Focal Adhesion-Independent Cell Migration Annu Rev Cell Dev Biol 2016 32 469 90 27501447
  48. [49]Ye L Wang C Hong L Sun N Chen D Chen S Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9 Cell Discov 2018 4 46 30062046 PMC6056518
  49. [50]Krause M Leslie JD Stewart M Lafuente EM Valderrama F Jagannathan R Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamics Dev Cell 2004 7 571 83 15469845
  50. [51]Gardel ML Schneider IC Aratyn-Schaus Y Waterman CM Mechanical integration of actin and adhesion dynamics in cell migration Annu Rev Cell Dev Biol 2010 26 315 33 19575647 PMC4437624
  51. [52]Etienne-Manneville S Microtubules in cell migration Annu Rev Cell Dev Biol 2013 29 471 99 23875648
  52. [53]Krause M Gautreau A Steering cell migration: lamellipodium dynamics and the regulation of directional persistence Nat Rev Mol Cell Biol 2014 15 577 90 25145849
  53. [54]Baschieri F Confalonieri S Bertalot G Di Fiore PP Dietmaier W Leist M Spatial control of Cdc42 signalling by a GM130-RasGRF complex regulates polarity and tumorigenesis Nat Commun 2014 5 4839 25208761 PMC4449154
  54. [55]Wang H Hong LJ Huang JY Jiang Q Tao RR Tan C P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy Cell Res 2015 25 674 90 25998681 PMC4456628
  55. [56]Leckband DE le Duc Q Wang N de Rooij J Mechanotransduction at cadherin-mediated adhesions Curr Opin Cell Biol 2011 23 523 30 21890337
  56. [57]Ross TD Coon BG Yun S Baeyens N Tanaka K Ouyang M Integrins in mechanotransduction Curr Opin Cell Biol 2013 25 613 8 23797029 PMC3757118
  57. [58]Jiang Q Gao Y Wang C Tao R Wu Y Zhan K Nitration of TRPM2 as a molecular switch induces autophagy during brain pericyte injury Antioxid Redox Signal 2017 27 1297 316 28292196
  58. [59]Hynes RO Integrins: bidirectional, allosteric signaling machines Cell 2002 110 673 87 12297042
  59. [60]Horton ER Byron A Askari JA Ng DHJ Millon-Fremillon A Robertson J Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly Nat Cell Biol 2015 17 1577 87 26479319 PMC4663675
  60. [61]Friedl P Gilmour D Collective cell migration in morphogenesis, regeneration and cancer Nat Rev Mol Cell Biol 2009 10 445 57 19546857
  61. [62]Hernandez-Vasquez MN Adame-Garcia SR Hamoud N Chidiac R Reyes-Cruz G Gratton JP Cell adhesion controlled by adhesion G protein-coupled receptor GPR124/ADGRA2 is mediated by a protein complex comprising intersectins and Elmo-Dock J Biol Chem 2017 292 12178 91 28600358 PMC5519368
  62. [63]Pollard TD Cooper JA Actin, a central player in cell shape and movement Science 2009 326 1208 12 19965462 PMC3677050
  63. [64]Vallon M Rohde F Janssen KP Essler M Tumor endothelial marker 5 expression in endothelial cells during capillary morphogenesis is induced by the small GTPase Rac and mediates contact inhibition of cell proliferation Exp Cell Res 2010 316 412 21 19853600
  64. [65]Vallon M Aubele P Janssen KP Essler M Thrombin-induced shedding of tumour endothelial marker 5 and exposure of its RGD motif are regulated by cell-surface protein disulfide-isomerase Biochem J 2012 441 937 44 22013897

Cited by

Sorted by citations

Authors
Chen, D.-Y., Sun, N.-H., Lu, Y.-P., Hong, L.-J., Cui, T.-T., Wang, C.-K., Chen, X.-H., Wang, S.-S., Feng, L.-L., Shi, W.-X., Fukunaga, K., Chen, Z., Lu, Y.-M., & Han, F.
Affiliations
  • Zhejiang University (CN)
  • Nanjing Medical University (CN)
  • Loma Linda University (US)
  • Tohoku University (JP)
Corresponding author
  • Chen, Zhong
  • Lu, Ying-Mei
  • Han, Feng
ORCID
Venue
Theranostics
Publisher
Ivyspring International Publisher
Published
2019
Volume
9
Issue
20
Pages
5937-5955
ISSN
1838-7640 (ISSN-L)
DOI
10.7150/thno.34168
PMC
PMC6735362
OpenAlex
W2972677877
Cited by
29
References
1
Citations by year
2026: 4 · 2025: 7 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 2 · 2020: 4 · 2019: 1
FWCI
1.21
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Theranostics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • The Scholars Repository - LLU (Loma Linda University) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
GPR124, pericytes, pericyte migration, filopodia formation, Cdc42 activation, ischemic injury, ischemic stroke, G protein-coupled receptors
MeSH terms
Animals, Cell Line, Brain Ischemia, Humans, Immunohistochemistry, Male, Plasmids, Wound Healing, Blotting, Western, Lentivirus, Cell Polarity, Pericytes, Focal Adhesions, Endothelial Cells, Receptors, G-Protein-Coupled, Immunoprecipitation, Mice, HEK293 Cells, Real-Time Polymerase Chain Reaction

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review