Current Progress in Gene Delivery Technology Based on Chemical Methods and Nano-Carriers
Open accessAbstract
Gene transfer methods are promising in the field of gene therapy. Current methods for gene transfer include three major groups: viral, physical and chemical methods. This review mainly summarizes development of several types of chemical methods for gene transfer in vitro and in vivo by means of nano-carriers like; calcium phosphates, lipids, and cationic polymers including chitosan, polyethylenimine, polyamidoamine dendrimers, and poly(lactide-co-glycolide). This review also briefly introduces applications of these chemical methods for gene delivery.
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Although polyplexes do not contain viral material, they are foreign materials and may lead to immune response by the host.
“Although these polyplexes do not contain any viral material, they are still foreign materials and they may lead to the immune response by the host [74].”
From Immune responses to gene editing by viral and non-viral delivery vectors used in retinal gene therapy (2022) · cites this paper as [74]
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References 272 · Cited by 388
Research works printed but not linked: 13
- [66]Zhi D Zhang S Wang B Zhao Y Yang B Yu S Transfection efficiency of cationic lipids with different hydrophobic domains in gene delivery Bioconjug Chem 2010 4 21 563 577 20121120
- [72]Nie Y Ji L Ding H Xie L Li L He B Wu Y Gu Z Cholesterol derivatives based charged liposomes for doxorubicin delivery: preparation, in vitro and in vivo characterization Theranostics 2012 2 11 1092 1103 23227125 PMC3516839
- [113]Lavertu M Methot S Tran-Khanh N Buschmann MD High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation Biomaterials 2006 27 27 4815 4824 16725196
- [118]Mao SR Sun W Kissel T Chitosan-based formulations for delivery of DNA and siRNA Adv Drug Deliver Rev 2010 1 62 12 27 19796660
- [123]Lavertu M Methot S Tran-Khanh N Buschmann MD High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation Biomaterials 2006 27 27 4815 4824 16725196
- [136]Vu L Ramos J Potta T Rege K Generation of a focused poly (amino ether) library: polymer-mediated transgene delivery and gold-nanorod based theranostic systems Theranostics 2012 2 12 1160 1173 23382773 PMC3563149
- [151]Fischer D Bieber T Li YX Elsasser HP Kissel T A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity Pharm Res 1999 8 16 1273 1279 10468031
- [171]Forrest ML Koerber JT Pack DW A degradable polyethylenimine derivative with low toxicity for highly efficient gene delivery Bioconjugate Chem 2003 5 14 934 940 13129396
- [210]Breunig M Lungwitz U Liebl R Goepferich A Breaking up the correlation between efficacy and toxicity for nonviral gene delivery Proc Natl Acad Sci U S A 2007 36 104 14454 14459 17726101 PMC1964826
- [218]Bhakta G Shrivastava A Maitra A Magnesium phosphate nanoparticles can be efficiently used in vitro and in vivo as non-viral vectors for targeted gene delivery. J. Biomed Nanotechnol 2009 1 5 106 114 20055113
- [240]Orrantia E Chang PL Intracellular-distribution of DNA internalized through calcium-phosphate precipitation Exp Cell Res 1990 2 190 170 174 2209719
- [270]Vu L Ramos J Potta T Rege K Generation of a focused poly(amino ether) library: polymer-mediated transgene delivery and gold-nanorod based theranostic systems Theranostics 2012 2 12 1160 1173 23382773 PMC3563149
- [277]Panje CM Wang DS Pysz MA Paulmurugan R Ren Y Tranquart F Tian L Willmann JK Ultrasound-mediated gene delivery with cationic versus neutral microbubbles: effect of DNA and microbubble dose on in vivo transfection efficiency Theranostics 2012 2 11 1078 1091 23227124 PMC3516840
- Authors
- Jin, L., Zeng, X., Liu, M., Deng, Y., & He, N.
- Affiliations
- State Key Laboratory of Digital Medical Engineering (CN)
- Nanjing Maternity and Child Health Care Hospital (CN)
- Corresponding author
- He, Nongyue
- Venue
- Theranostics
- Publisher
- Ivyspring International Publisher
- Published
- 2014
- Volume
- 4
- Issue
- 3
- Pages
- 240-255
- ISSN
- 1838-7640 (ISSN-L)
- PMC
- PMC3915088
- OpenAlex
- W2150092048
- Cited by
- 388
- References
- 272
- Citations by year
- 2026: 10 · 2025: 14 · 2024: 17 · 2023: 20 · 2022: 34 · 2021: 30 · 2020: 36 · 2019: 39 · 2018: 41 · 2017: 56 · 2016: 38 · 2015: 38 · 2014: 15
- FWCI
- 4.62
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Theranostics (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31201003, 61271056, 2014CB744501)
- China Postdoctoral Science Foundation (20100471362)
- Southeast University
- State Key Laboratory of Bioelectronics
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- gene delivery, nanocarriers, calcium phosphate, cationic polymers, chitosan, polyethyleneimine, PAMAM dendrimers, poly(lactic-co-glycolic acid), gene therapy
- MeSH terms
- Animals, Humans, Liposomes, Polymers, Genetic Therapy, Gene Transfer Techniques, Nanoparticles
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