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Cited by 1Bacterial Genetics and BiotechnologyEscherichia coli research studiesMicrobial metabolism and enzyme function

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Coordinated regulation of chemotaxis and resistance to copper by CsoR in Pseudomonas putida

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He, Meina, Tao, Yongxin, Mu, Kexin, Feng, Haoqi, Fan, Ying, Liu, Tong, Huang, Qiaoyun, Xiao, Yujie, Chen, Wenli·2025·eLife
DOI 10.7554/elife.100914PMC PMC11978298

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Abstract

Copper is an essential enzyme cofactor in bacteria, but excess copper is highly toxic. Bacteria can cope with copper stress by increasing copper resistance and initiating chemorepellent response. However, it remains unclear how bacteria coordinate chemotaxis and resistance to copper. By screening proteins that interacted with the chemotaxis kinase CheA, we identified a copper-binding repressor CsoR that interacted with CheA in Pseudomonas putida . CsoR interacted with the HPT (P1), Dimer (P3), and HATPase_c (P4) domains of CheA and inhibited CheA autophosphorylation, resulting in decreased chemotaxis. The copper-binding of CsoR weakened its interaction with CheA, which relieved the inhibition of chemotaxis by CsoR. In addition, CsoR bound to the promoter of copper-resistance genes to inhibit gene expression, and copper-binding released CsoR from the promoter, leading to increased gene expression and copper resistance. P. putida cells exhibited a chemorepellent response to copper in a CheA-dependent manner, and CsoR inhibited the chemorepellent response to copper. Besides, the CheA-CsoR interaction also existed in proteins from several other bacterial species. Our results revealed a mechanism by which bacteria coordinately regulated chemotaxis and resistance to copper by CsoR.

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Metadata

Authors
He, M., Tao, Y., Mu, K., Feng, H., Fan, Y., Liu, T., Huang, Q., Xiao, Y., & Chen, W.
Affiliations
Huazhong Agricultural University (CN)
Corresponding author
  • Xiao, Yujie
  • Chen, Wenli
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2025-04-08
Volume
13
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.100914
PMC
PMC11978298
OpenAlex
W4402359928
Cited by
1
References
4
Citations by year
2025: 1
Type
posted content
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Key Research and Development Program of China (2020YFC1806803)
  • Hubei Provincial Natural Science Foundation of China (2024AFB691)
  • National Natural Science Foundation of China (31900054)
  • Fundamental Research Funds for the Central Universities (2662022SKQD002)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
Keywords
copper resistance, chemotaxis, CheA kinase, autophosphorylation inhibition, promoter repression, copper stress, Pseudomonas putida, transcriptional regulation
MeSH terms
Methyl-Accepting Chemotaxis Proteins, Bacterial Proteins, Chemotaxis, Copper, Promoter Regions, Genetic, Protein Binding, Repressor Proteins, Gene Expression Regulation, Bacterial, Pseudomonas putida, Drug Resistance, Bacterial

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